Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism
UNSPECIFIED. (2002) Detection and localization of two hydrogenases in Methylococcus capsulatus (Bath) and their potential role in methane metabolism. ARCHIVES OF MICROBIOLOGY, 177 (2). pp. 167-172. ISSN 0302-8933Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s00203-001-0372-4
Methylococcus capsulatus (Bath) was shown to contain two distinct hydrogenases, a soluble hydrogenase and a membrane-bound hydrogenase. This is the first report of a membrane-bound hydrogenase in methanotrophs. Both enzymes were expressed apparently constitutively under normal growth conditions. The soluble hydrogenase was capable of reducing NAD(+) with molecular hydrogen. The activities of both soluble and particulate methane monooxygenases could be driven by molecular hydrogen. This confirmed that molecular hydrogen could be used as a source of reducing power for methane oxidation. Hydrogen-driven methane monooxygenase activities tolerated elevated temperatures and moderate oxygen concentrations. The significance of these findings for biotechnological applications of methanotrophs is discussed.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||ARCHIVES OF MICROBIOLOGY|
|Number of Pages:||6|
|Page Range:||pp. 167-172|
Actions (login required)