5-methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis
UNSPECIFIED. (2002) 5-methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis. Journal of Molecular Biology, 316 (1). pp. 89-100. ISSN 0022-2836Full text not available from this repository.
Official URL: http://dx.doi.org/10.1006/jmbi.2001.5317
We have identified and characterised in several strains of Mycobacterium tuberculosis a new 5-methylthiopentose substituent on lipoarabinomannan (LAM). The 5-methylthiopentose was initially observed in heteronuclear H-1-C-13-NMR spectra of intact, C-13-enriched LAM. Oligosaccharides carrying this substituent were released from C-13-enriched LAM and from unlabelled LAM using an endo-arabinanase from Cellulomonas gellida. The presence of the methylthio group in these oligosaccharides was established using NMR, high-resolution Fourier-transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry using a Q-TOF mass spectrometer. The 5-methylthiopentose is linked to a terminal mannose in the cap structures of these oligosaccharides as evidenced by tandem mass spectrometry and by NMR. We suggest interference with the signal transduction mechanisms of infected macrophages as a possible function for this newly discovered LAM substituent. (C) 2002 Elsevier Science Ltd.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry|
|Journal or Publication Title:||Journal of Molecular Biology|
|Publisher:||ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD|
|Date:||8 February 2002|
|Number of Pages:||12|
|Page Range:||pp. 89-100|
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