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Sulfonated copolymers as heparin-mimicking stabilizer of fibroblast growth factor : size, architecture, and monomer distribution effects
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Bray, Caroline, Gurnani, Pratik, Mansfield, Edward D. H., Peltier, Raoul and Perrier, Sébastien (2019) Sulfonated copolymers as heparin-mimicking stabilizer of fibroblast growth factor : size, architecture, and monomer distribution effects. Biomacromolecules, 20 (1). pp. 285-293. doi:10.1021/acs.biomac.8b01451
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WRAP-sulfonated-copolymers-heparin-mimicking-stabilizer-Perrier-2018.pdf - Accepted Version - Requires a PDF viewer. Download (1644Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.biomac.8b01451
Abstract
Fibroblast growth factors (FGF) are involved in a wide range of biological processes such as cell proliferation and differentiation. In living organisms, the binding of FGF to its receptors are mediated through electrostatic interactions between FGF and naturally occurring heparin. Despite its prevalent use in medicine, heparin carries notable limitations, namely; its extraction from natural sources (expensive, low yield and extensive purification), viral contamination, and batch-to-batch heterogeneity. In this work a range of synthetic homopolymers and copolymers of sodium 2-acrylamido-2-methylpropane sulfonate (AMPS®) were evaluated as potential FGF stabilisers. This was studied by measuring the proliferation of BaF3-FR1c cells, as a model assay, and the results will be compared with the natural stabilisation and activation of FGF by heparin. This study explores the structure-activity relationship of these polysulfonated polymers with a focus on the effect of molecular weight, co-monomer type, charge dispersion and polymer architecture on protein stabilisation.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Chemistry | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Fibroblasts, Copolymers, Heparin | |||||||||||||||
| Journal or Publication Title: | Biomacromolecules | |||||||||||||||
| Publisher: | American Chemical Society | |||||||||||||||
| ISSN: | 1525-7797 | |||||||||||||||
| Official Date: | 2019 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 24 January 2019 | |||||||||||||||
| Volume: | 20 | |||||||||||||||
| Number: | 1 | |||||||||||||||
| Page Range: | pp. 285-293 | |||||||||||||||
| DOI: | 10.1021/acs.biomac.8b01451 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Publisher Statement: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.biomac.8b01451 | |||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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