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An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens
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Bianchet, Chantal, Wong, Aloysius, Quaglia, Mara, Alqurashi, May, Gehring, Chris, Ntoukakis, Vardis and Pasqualini, Stefania (2019) An Arabidopsis thaliana leucine-rich repeat protein harbors an adenylyl cyclase catalytic center and affects responses to pathogens. Journal of Plant Physiology, 232 . pp. 12-22. doi:10.1016/j.jplph.2018.10.025
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Official URL: http://dx.doi.org/10.1016/j.jplph.2018.10.025
Abstract
Adenylyl cyclases (ACs) catalyze the formation of the second messenger cAMP from ATP. Here we report the characterization of an Arabidopsis thaliana leucine-rich repeat (LRR) protein (At3g14460; AtLRRAC1) as an adenylyl cyclase. Using an AC-specific search motif supported by computational assessments of protein models we identify an AC catalytic center within the N-terminus and demonstrate that AtLRRAC1 can generate cAMP in vitro. Knock-out mutants of AtLRRAC1 have compromised immune responses to the biotrophic fungus Golovinomyces orontii and the hemibiotrophic bacteria Pseudomonas syringae, but not against the necrotrophic fungus Botrytis cinerea. These findings are consistent with a role of cAMP-dependent pathways in the defense against biotrophic and hemibiotrophic plant pathogens.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Adenylate cyclase, Arabidopsis thaliana, Botrytis cinerea, Pseudomonas syringae | ||||||||||||||||||
| Journal or Publication Title: | Journal of Plant Physiology | ||||||||||||||||||
| Publisher: | Elsevier Science BV | ||||||||||||||||||
| ISSN: | 0176-1617 | ||||||||||||||||||
| Official Date: | January 2019 | ||||||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 24 January 2019 | ||||||||||||||||||
| Volume: | 232 | ||||||||||||||||||
| Page Range: | pp. 12-22 | ||||||||||||||||||
| DOI: | 10.1016/j.jplph.2018.10.025 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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