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Data for Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage
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Mitchell, Daniel A., Fayter, Alice, Deller, Robert C., Hasan, Muhammad, Gutierrez-Marcos, José F. and Gibson, Matthew I. (2019) Data for Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage. [Dataset]
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Abstract
Proteins are ubiquitous in molecular biotechnology, biotechnology and as therapeutics, but there are significant challenges in their storage and distribution, with freezing often required. This is traditionally achieved by the addition of cryoprotective agents such as glycerol (or trehalose) or covalent modification of mutated proteins with cryoprotectants. Here, ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are used synergistically with poly(ethylene glycol) as an alternative to glycerol. The primary mechanism of action appears to be preventing irreversible aggregation due to ice growth. The polymer formulation is successfully used to cryopreserve a range of important proteins including insulin, Taq DNA polymerase and an IgG antibody. The polymers do not require covalent conjugation, nor modification of the protein and are already used in a wide range of biomedical applications, which will facilitate translation to a range of biologics.
| Item Type: | Dataset | |||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Science > Life Sciences (2010- ) Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Type of Data: | Tabular | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Polymers, Antifreeze proteins, Glycerin, Trehalose | |||||||||||||||
| Journal or Publication Title: | Materials Horizons | |||||||||||||||
| Publisher: | University of Warwick / Royal Society of Chemistry (RSC) | |||||||||||||||
| ISSN: | 2051-6355 | |||||||||||||||
| Official Date: | 1 February 2019 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 28 November 2018 | |||||||||||||||
| Status: | Not Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Media of Output: | .docx .xlsx | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| Description: | Data for the protein freezing experiments divided into two worksheets are included Worksheet 2 contains GFP and Insulin. Worksheet 1 contains all other proteins |
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| RIOXX Funder/Project Grant: |
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