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Data for Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage

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Mitchell, Daniel A., Fayter, Alice, Deller, Robert C., Hasan, Muhammad, Gutierrez-Marcos, José F. and Gibson, Matthew I. (2019) Data for Ice-recrystallization inhibiting polymers protect proteins against freeze-stress and enable glycerol-free cryostorage. [Dataset]

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Abstract

Proteins are ubiquitous in molecular biotechnology, biotechnology and as therapeutics, but there are significant challenges in their storage and distribution, with freezing often required. This is traditionally achieved by the addition of cryoprotective agents such as glycerol (or trehalose) or covalent modification of mutated proteins with cryoprotectants. Here, ice recrystallization inhibiting polymers, inspired by antifreeze proteins, are used synergistically with poly(ethylene glycol) as an alternative to glycerol. The primary mechanism of action appears to be preventing irreversible aggregation due to ice growth. The polymer formulation is successfully used to cryopreserve a range of important proteins including insulin, Taq DNA polymerase and an IgG antibody. The polymers do not require covalent conjugation, nor modification of the protein and are already used in a wide range of biomedical applications, which will facilitate translation to a range of biologics.

Item Type: Dataset
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Divisions: Faculty of Science > Chemistry
Faculty of Science > Life Sciences (2010- )
Faculty of Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Type of Data: Tabular
Library of Congress Subject Headings (LCSH): Polymers, Antifreeze proteins, Glycerin, Trehalose
Journal or Publication Title: Materials Horizons
Publisher: University of Warwick / Royal Society of Chemistry (RSC)
ISSN: 2051-6355
Official Date: 1 February 2019
Dates:
DateEvent
1 February 2019Published
8 November 2018Available
19 October 2018Accepted
Status: Not Peer Reviewed
Publication Status: Published
Media of Output: .docx .xlsx
Access rights to Published version: Open Access
Description:

Data for the protein freezing experiments divided into two worksheets are included

Worksheet 2 contains GFP and Insulin. Worksheet 1 contains all other proteins

RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIED[RS] Royal Societyhttp://dx.doi.org/10.13039/501100000288
EP/F500378/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
638661European Research Councilhttp://viaf.org/viaf/130022607
EP/R511808/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
Related URLs:
  • Related item in WRAP
  • https://doi.org/10.1039/C8MH00727F

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