Čapek, Daniel, Smutny, Michael, Tichy, Alexandra-Madelaine, Morri, Maurizio, Janovjak, Harald and Heisenberg, Carl-Philipp (2019) Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife, 8 . e42093. doi:10.7554/elife.42093

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Abstract

10.7554/eLife.42093.001Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School
SWORD Depositor:	Library Publications Router
Library of Congress Subject Headings (LCSH):	Cell migration, Epithelial cells
Journal or Publication Title:	eLife
Publisher:	eLife Sciences Publications, Ltd
ISSN:	2050-084X
Official Date:	6 February 2019
Dates:	Date Event 6 February 2019 Published 16 January 2019 Available 14 January 2019 Accepted
Volume:	8
Article Number:	e42093
DOI:	10.7554/elife.42093
Status:	Peer Reviewed
Publication Status:	Published
Publisher Statement:	** From eLife via Jisc Publications Router. ** History: received 17-09-2018; collection 2019; accepted 14-01-2019; publication 16-01-2019. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/
Access rights to Published version:	Open Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID MECSPEC European Research Council http://viaf.org/viaf/130022607 UNSPECIFIED State Government of Victoria http://dx.doi.org/10.13039/501100004752 Australia Partnership Laboratory (EMBL) Australia. Government UNSPECIFIED National CollaborativeResearch Infrastructure Strategy (NCRIS) Australia. Government UNSPECIFIED
Related URLs:	Other
Contributors:	Contribution Name Contributor ID UNSPECIFIED Stainier, Didier Y UNSPECIFIED UNSPECIFIED Stainier, Didier Y UNSPECIFIED

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