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Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration
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Čapek, Daniel, Smutny, Michael, Tichy, Alexandra-Madelaine, Morri, Maurizio, Janovjak, Harald and Heisenberg, Carl-Philipp (2019) Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. eLife, 8 . e42093. doi:10.7554/elife.42093
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Official URL: https://doi.org/10.7554/elife.42093
Abstract
10.7554/eLife.42093.001Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QH Natural history | |||||||||||||||
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cell migration, Epithelial cells | |||||||||||||||
| Journal or Publication Title: | eLife | |||||||||||||||
| Publisher: | eLife Sciences Publications, Ltd | |||||||||||||||
| ISSN: | 2050-084X | |||||||||||||||
| Official Date: | 6 February 2019 | |||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 4 April 2019 | |||||||||||||||
| Volume: | 8 | |||||||||||||||
| Article Number: | e42093 | |||||||||||||||
| DOI: | 10.7554/elife.42093 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Publisher Statement: | ** From eLife via Jisc Publications Router. ** History: received 17-09-2018; collection 2019; accepted 14-01-2019; publication 16-01-2019. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ | |||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||
| RIOXX Funder/Project Grant: |
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