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Scaffolding of enzymes on virus nanoarrays : effects of confinement and virus organization on biocatalysis
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Patel, Anisha N., Anne, Agnès, Chovin, Arnaud, Demaille, Christophe, Grelet, Eric, Michon, Thierry and Taofifenua, Cécilia (2017) Scaffolding of enzymes on virus nanoarrays : effects of confinement and virus organization on biocatalysis. Small, 13 (13). 1603163. doi:10.1002/smll.201603163 ISSN 1613-6810.
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Official URL: http://dx.doi.org/10.1002/smll.201603163
Abstract
Organizing active enzyme molecules on nanometer‐sized scaffolds is a promising strategy for designing highly efficient supported catalytic systems for biosynthetic and sensing applications. This is achieved by designing model nanoscale enzymatic platforms followed by thorough analysis of the catalytic activity. Herein, the virus fd bacteriophage is considered as an enzyme nanocarrier to study the scaffolding effects on enzymatic activity. Nanoarrays of randomly oriented, or directionally patterned, fd bacteriophage virus are functionalized with the enzyme glucose oxidase (GOx), using an immunological assembly strategy, directly on a gold electrode support. The scaffolding process on the virus capsid is monitored in situ by AFM (atomic force microscopy) imaging, while cyclic voltammetry is used to interrogate the catalytic activity of the resulting functional GOx‐fd nanoarrays. Kinetic analysis reveals the ability to modulate the activity of GOx via nanocarrier patterning. The results evidence, for the first time, enhancement of the enzymatic activity due to scaffolding on a filamentous viral particle.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||||
Journal or Publication Title: | Small | ||||||
Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | ||||||
ISSN: | 1613-6810 | ||||||
Official Date: | April 2017 | ||||||
Dates: |
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Volume: | 13 | ||||||
Number: | 13 | ||||||
Article Number: | 1603163 | ||||||
DOI: | 10.1002/smll.201603163 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Restricted or Subscription Access |
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