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Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)
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UNSPECIFIED (2001) Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath). FEMS MICROBIOLOGY LETTERS, 205 (2). pp. 203-207. ISSN 0378-1097.
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Abstract
The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A Delta hupSL deletion mutant of Me. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QR Microbiology | ||||
Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS | ||||
Publisher: | ELSEVIER SCIENCE BV | ||||
ISSN: | 0378-1097 | ||||
Official Date: | 18 December 2001 | ||||
Dates: |
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Volume: | 205 | ||||
Number: | 2 | ||||
Number of Pages: | 5 | ||||
Page Range: | pp. 203-207 | ||||
Publication Status: | Published |
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