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Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath)

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UNSPECIFIED (2001) Molecular characterization of structural genes coding for a membrane bound hydrogenase in Methylococcus capsulatus (Bath). FEMS MICROBIOLOGY LETTERS, 205 (2). pp. 203-207. ISSN 0378-1097

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Abstract

The first gene cluster encoding for a membrane bound [NiFe] hydrogenase from a methanotroph, Methylococcus capsulatus (Bath), was cloned and sequenced. The cluster consisted of the structural genes hupS and hupL and accessory genes hupE, hupC and hupD. A Delta hupSL deletion mutant of Me. capsulatus was constructed by marker exchange mutagenesis. Membrane associated hydrogenase activity disappeared. The membrane associated hydrogenase appeared to have a hydrogen uptake function in vivo. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Journal or Publication Title: FEMS MICROBIOLOGY LETTERS
Publisher: ELSEVIER SCIENCE BV
ISSN: 0378-1097
Date: 18 December 2001
Volume: 205
Number: 2
Number of Pages: 5
Page Range: pp. 203-207
Publication Status: Published
URI: http://wrap.warwick.ac.uk/id/eprint/11395

Data sourced from Thomson Reuters' Web of Knowledge

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