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The accumulation of the influenza virus nucleoprotein in the nuclei of 'xenopus' oocytes

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Davey, John (1984) The accumulation of the influenza virus nucleoprotein in the nuclei of 'xenopus' oocytes. PhD thesis, University of Warwick.

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Official URL: http://webcat.warwick.ac.uk/record=b1464807~S1

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Abstract

This work concerns the identification of the information controlling the accumulation of the influenza virus nucleoprotein (NP) in the nuclei of Xenopus oocytes.
The NP accumulates in the nuclei of Xenopus oocytes whether introduced into the oocytes as the protein itself or encoded in RNA or DNA. Since no other influenza virus components are present in the oocytes injected with DNA, this accumulation appears to be a property of the NP itself.
In vitro mutagenesis of the cloned NP cDNA has then been used to identify which regions of the protein are important in its nuclear accumulation. Mutant proteins lacking amino acids 327-345 of wild-type NP enter the nucleus but do not accumulate there to the same extent as the wild-type protein, suggesting that this region has a role in nuclear accumulation. This proposed location is strengthened by studies involving the production of fusion proteins in which various amino- terminal sequences of the NP gene are fused to the complete chimpanzee ꙋ1-globin sequence: when globin cDNA is injected into and expressed in oocytes the protein remains in the cytosol, however when the globin cDNA is fused to a portion of NP cDNA which includes the region encoding amino acids 327-345 the resulting fusion protein enters and accumulates in the nucleus. Fusion proteins lacking this region of the NP enter but do not accumulate in the nucleus

Item Type: Thesis (PhD)
Subjects: Q Science > QR Microbiology
Library of Congress Subject Headings (LCSH): Microbiology, Nucleoproteins, Influenza viruses, Molecular virology, Molecular biology, Cytology, Genetics
Official Date: October 1984
Dates:
DateEvent
October 1984Submitted
Institution: University of Warwick
Theses Department: Department of Biological Sciences
Thesis Type: PhD
Publication Status: Unpublished
Supervisor(s)/Advisor: Dimmock, N. J., Colman, Alan
Sponsors: Science and Engineering Research Council (Great Britain)
Extent: xxix, 345 leaves : illustrations
Language: eng

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