Richardson, Sarah, Rawlings, Thomas, Muter, Joanne, Walker, Marc, Brosens, Jan J., Cameron, Neil R. and Eissa, Ahmed M. (2019) Covalent attachment of fibronectin onto emulsion‐templated porous polymer scaffolds enhances human endometrial stromal cell adhesion, infiltration, and function. Macromolecular Bioscience, 19 (2). 1800351. doi:10.1002/mabi.201800351

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Abstract

A novel strategy for the surface functionalization of emulsion‐templated highly porous (polyHIPE) materials as well as its application to in vitro 3D cell culture is presented. A heterobifunctional linker that consists of an amine‐reactive N‐hydroxysuccinimide ester and a photoactivatable nitrophenyl azide, N‐sulfosuccinimidyl‐6‐(4′‐azido‐2′‐nitrophenylamino)hexanoate (sulfo‐SANPAH), is utilized to functionalize polyHIPE surfaces. The ability to conjugate a range of compounds (6‐aminofluorescein, heptafluorobutylamine, poly(ethylene glycol) bis‐amine, and fibronectin) to the polyHIPE surface is demonstrated using fluorescence imaging, FTIR spectroscopy, and X‐ray photoelectron spectroscopy. Compared to other existing surface functionalization methods for polyHIPE materials, this approach is facile, efficient, versatile, and benign. It can also be used to attach biomolecules to polyHIPE surfaces including cell adhesion‐promoting extracellular matrix proteins. Cell culture experiments demonstrated that the fibronectin‐conjugated polyHIPE scaffolds improve the adhesion and function of primary human endometrial stromal cells. It is believed that this approach can be employed to produce the next generation of polyHIPE scaffolds with tailored surface functionality, enhancing their application in 3D cell culture and tissue engineering whilst broadening the scope of applications to a wider range of cell types.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science > Chemistry Faculty of Science > Engineering Faculty of Science > Physics Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Fibronectins, Endometrium
Journal or Publication Title:	Macromolecular Bioscience
Publisher:	Wiley - V C H Verlag GmbH & Co. KGaA
ISSN:	1616-5187
Official Date:	February 2019
Dates:	Date Event February 2019 Published 13 December 2018 Available 13 December 2018 Accepted
Date of first compliant deposit:	6 March 2019
Volume:	19
Number:	2
Article Number:	1800351
DOI:	10.1002/mabi.201800351
Status:	Peer Reviewed
Publication Status:	Published
Publisher Statement:	This is the peer reviewed version of the following article: S. A. Richardson, T. M. Rawlings, J. Muter, M. Walker, J. J. Brosens, N. R. Cameron, A. M. Eissa, Macromol. Biosci. 2019, 1800351. https://doi.org/10.1002/mabi.201800351, which has been published in final form at https://doi.org/10.1002/mabi.201800351. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Access rights to Published version:	Restricted or Subscription Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID UNSPECIFIED University of Warwick http://dx.doi.org/10.13039/501100000741 UNSPECIFIED Monash University http://dx.doi.org/10.13039/501100001779 UNSPECIFIED [NIHR] National Institute for Health Research http://dx.doi.org/10.13039/501100000272

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