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Covalent attachment of fibronectin onto emulsion‐templated porous polymer scaffolds enhances human endometrial stromal cell adhesion, infiltration, and function
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Richardson, Sarah, Rawlings, Thomas M., Muter, Joanne, Walker, Marc, Brosens, Jan J., Cameron, Neil R. and Eissa, Ahmed M. (2019) Covalent attachment of fibronectin onto emulsion‐templated porous polymer scaffolds enhances human endometrial stromal cell adhesion, infiltration, and function. Macromolecular Bioscience, 19 (2). 1800351. doi:10.1002/mabi.201800351 ISSN 1616-5187.
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WRAP-covalent-attachment-fibronectin-emulsion‐templated-porous-polymer-scaffolds-Eissa-2019.pdf - Accepted Version - Requires a PDF viewer. Download (1431Kb) | Preview |
Official URL: http://dx.doi.org/10.1002/mabi.201800351
Abstract
A novel strategy for the surface functionalization of emulsion‐templated highly porous (polyHIPE) materials as well as its application to in vitro 3D cell culture is presented. A heterobifunctional linker that consists of an amine‐reactive N‐hydroxysuccinimide ester and a photoactivatable nitrophenyl azide, N‐sulfosuccinimidyl‐6‐(4′‐azido‐2′‐nitrophenylamino)hexanoate (sulfo‐SANPAH), is utilized to functionalize polyHIPE surfaces. The ability to conjugate a range of compounds (6‐aminofluorescein, heptafluorobutylamine, poly(ethylene glycol) bis‐amine, and fibronectin) to the polyHIPE surface is demonstrated using fluorescence imaging, FTIR spectroscopy, and X‐ray photoelectron spectroscopy. Compared to other existing surface functionalization methods for polyHIPE materials, this approach is facile, efficient, versatile, and benign. It can also be used to attach biomolecules to polyHIPE surfaces including cell adhesion‐promoting extracellular matrix proteins. Cell culture experiments demonstrated that the fibronectin‐conjugated polyHIPE scaffolds improve the adhesion and function of primary human endometrial stromal cells. It is believed that this approach can be employed to produce the next generation of polyHIPE scaffolds with tailored surface functionality, enhancing their application in 3D cell culture and tissue engineering whilst broadening the scope of applications to a wider range of cell types.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QH Natural history | ||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Engineering > Engineering Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Fibronectins, Endometrium | ||||||||||||
| Journal or Publication Title: | Macromolecular Bioscience | ||||||||||||
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA | ||||||||||||
| ISSN: | 1616-5187 | ||||||||||||
| Official Date: | February 2019 | ||||||||||||
| Dates: |
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| Volume: | 19 | ||||||||||||
| Number: | 2 | ||||||||||||
| Article Number: | 1800351 | ||||||||||||
| DOI: | 10.1002/mabi.201800351 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Reuse Statement (publisher, data, author rights): | This is the peer reviewed version of the following article: S. A. Richardson, T. M. Rawlings, J. Muter, M. Walker, J. J. Brosens, N. R. Cameron, A. M. Eissa, Macromol. Biosci. 2019, 1800351. https://doi.org/10.1002/mabi.201800351, which has been published in final form at https://doi.org/10.1002/mabi.201800351. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| Date of first compliant deposit: | 6 March 2019 | ||||||||||||
| Date of first compliant Open Access: | 13 December 2019 | ||||||||||||
| RIOXX Funder/Project Grant: |
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