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Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides

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Rihtman, Branko, Bowman-Grahl, Sabine, Millard, Andrew, Corrigan, Rebecca M., Clokie, Martha R. J. and Scanlan, David J. (2019) Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides. Environmental Microbiology Reports, 11 (3). pp. 448-445. doi:10.1111/1758-2229.12741

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Official URL: http://dx.doi.org/10.1111/1758-2229.12741

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Abstract

Bacteriophage possess a variety of auxiliary metabolic genes (AMGs) of bacterial origin. These proteins enable them to maximise infection efficiency, subverting bacterial metabolic processes for the purpose of viral genome replication and synthesis of the next generation of virion progeny. Here, we examined the enzymatic activity of a cyanophage MazG protein – a putative pyrophosphohydrolase previously implicated in regulation of the stringent response via reducing levels of the central alarmone molecule (p)ppGpp. We demonstrate however, that the purified viral MazG shows no binding or hydrolysis activity against (p)ppGpp. Instead, dGTP and dCTP appear to be the preferred substrates of this protein, consistent with a role preferentially hydrolysing deoxyribonucleotides from the high GC content host Synechococcus genome. This showcases a new example of the fine‐tuned nature of viral metabolic processes.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology > QR355 Virology
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Bacteriophages, Viral genomes, Hydrolysis
Journal or Publication Title: Environmental Microbiology Reports
Publisher: Wiley-Blackwell Publishing Ltd.
ISSN: 1758-2229
Official Date: June 2019
Dates:
DateEvent
June 2019Published
27 February 2019Available
26 February 2019Accepted
Volume: 11
Number: 3
Page Range: pp. 448-445
DOI: 10.1111/1758-2229.12741
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: "This is the peer reviewed version of the following article:Rihtman, B., Bowman‐Grahl, S. , Millard, A. , Corrigan, R. M., Clokie, M. R. and Scanlan, D. J. (2019), Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides. Environmental Microbiology Reports. Accepted Author Manuscript. doi:10.1111/1758-2229.12741, which has been published in final form at http://dx.doi.org/10.1111/1758-2229.12741 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions."
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
Chancellor’s ScholarshipUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
NE/J02273X/1 [NERC] Natural Environment Research Councilhttp://dx.doi.org/10.13039/501100000270
NE/N003241/1[NERC] Natural Environment Research Councilhttp://dx.doi.org/10.13039/501100000270
UNSPECIFIED[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MR/L015080/1Metabolic Support UKUNSPECIFIED

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