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Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides
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Rihtman, Branko, Bowman-Grahl, Sabine, Millard, Andrew, Corrigan, Rebecca M., Clokie, Martha R. J. and Scanlan, David J. (2019) Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides. Environmental Microbiology Reports, 11 (3). pp. 448-445. doi:10.1111/1758-2229.12741
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WRAP-cyanophage-pyrophosphohydrolase-magic-spot-Rihtman-2019.pdf - Accepted Version - Requires a PDF viewer. Download (2468Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/1758-2229.12741
Abstract
Bacteriophage possess a variety of auxiliary metabolic genes (AMGs) of bacterial origin. These proteins enable them to maximise infection efficiency, subverting bacterial metabolic processes for the purpose of viral genome replication and synthesis of the next generation of virion progeny. Here, we examined the enzymatic activity of a cyanophage MazG protein – a putative pyrophosphohydrolase previously implicated in regulation of the stringent response via reducing levels of the central alarmone molecule (p)ppGpp. We demonstrate however, that the purified viral MazG shows no binding or hydrolysis activity against (p)ppGpp. Instead, dGTP and dCTP appear to be the preferred substrates of this protein, consistent with a role preferentially hydrolysing deoxyribonucleotides from the high GC content host Synechococcus genome. This showcases a new example of the fine‐tuned nature of viral metabolic processes.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | Q Science > QR Microbiology > QR355 Virology | ||||||||||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Bacteriophages, Viral genomes, Hydrolysis | ||||||||||||||||||
| Journal or Publication Title: | Environmental Microbiology Reports | ||||||||||||||||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||||||||||||||||
| ISSN: | 1758-2229 | ||||||||||||||||||
| Official Date: | June 2019 | ||||||||||||||||||
| Dates: |
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| Volume: | 11 | ||||||||||||||||||
| Number: | 3 | ||||||||||||||||||
| Page Range: | pp. 448-445 | ||||||||||||||||||
| DOI: | 10.1111/1758-2229.12741 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Publisher Statement: | "This is the peer reviewed version of the following article:Rihtman, B., Bowman‐Grahl, S. , Millard, A. , Corrigan, R. M., Clokie, M. R. and Scanlan, D. J. (2019), Cyanophage MazG is a pyrophosphohydrolase but unable to hydrolyse magic spot nucleotides. Environmental Microbiology Reports. Accepted Author Manuscript. doi:10.1111/1758-2229.12741, which has been published in final form at http://dx.doi.org/10.1111/1758-2229.12741 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions." | ||||||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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