UNSPECIFIED (2001) TCR dynamics on the surface of living T cells. INTERNATIONAL IMMUNOLOGY, 13 (12). pp. 1525-1532. ISSN 0953-8178

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Abstract

T lymphocyte activation by specific antigen requires prolonged TCR occupancy and sustained signaling. This is accomplished by the formation of a specialized signaling domain, the immunological synapse, at the T cell-antigen-presenting cell contact site. Surface receptors and signaling components are progressively recruited into this domain where they are organized in defined three-dimensional structures. To better understand how TCR are supplied to the signaling domain during the activation process, we measured (using confocal microscopy and photo-bleaching recovery techniques) lateral mobility of GFP-tagged TCR on living Jurkat cell surface. We show that: (i) surface-expressed TCR exhibit an intrinsic, actin cytoskeleton-independent, lateral mobility which allows them to passively diffuse over the entire T cell surface within similar to 60 min and (ii) non-stimulated TCR rapidly enter the signaling domain. Our results indicate that TCR lateral mobility per se is sufficient to ensure TCR supply to the immunological synapse in the course of sustained T cell activation.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR180 Immunology
Journal or Publication Title:	INTERNATIONAL IMMUNOLOGY
Publisher:	OXFORD UNIV PRESS
ISSN:	0953-8178
Date:	December 2001
Volume:	13
Number:	12
Number of Pages:	8
Page Range:	pp. 1525-1532
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/11477

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