The Library
EBV-encoded miRNAs target ATM-mediated response in nasopharyngeal carcinoma
Tools
Tools
Tools
Lung, Raymond W-M., Hau, Pok-Man, Yu, Ken H-O., Yip, Kevin Y., Tong, Joanna H-M., Chak, Wing-Po, Chan, Anthony W-H., Lam, Ka-Hei, Lo, Angela Kwok-Fung, Tin, Edith K-Y., Chau, Shuk-Ling, Pang, Jesse C-S., Kwan, Johnny S-H., Busson, Pierre, Young, Lawrence S., Yap, Lee-Fah, Tsao, Sai-Wah, To, Ka-Fai and Lo, Kwok-Wai (2018) EBV-encoded miRNAs target ATM-mediated response in nasopharyngeal carcinoma. The Journal of Pathology, 244 (4). pp. 394-407. doi:10.1002/path.5018
|
PDF
WRAP-EBV-encoded-miRNAs-target-ATM-mediated-response-nasopharyngeal-carcinoma-Young-2017.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2580Kb) | Preview |
Official URL: http://dx.doi.org/10.1002/path.5018
Abstract
Nasopharyngeal carcinoma (NPC) is a highly invasive epithelial malignancy that is prevalent in southern China and Southeast Asia. It is consistently associated with latent Epstein–Barr virus (EBV) infection. In NPC, miR‐BARTs, the EBV‐encoded miRNAs derived from BamH1‐A rightward transcripts, are abundantly expressed and contribute to cancer development by targeting various cellular and viral genes. In this study, we establish a comprehensive transcriptional profile of EBV‐encoded miRNAs in a panel of NPC patient‐derived xenografts and an EBV‐positive NPC cell line by small RNA sequencing. Among the 40 miR‐BARTs, predominant expression of 22 miRNAs was consistently detected in these tumors. Among the abundantly expressed EBV‐miRNAs, BART5‐5p, BART7‐3p, BART9‐3p, and BART14‐3p could negatively regulate the expression of a key DNA double‐strand break (DSB) repair gene, ataxia telangiectasia mutated (ATM), by binding to multiple sites on its 3'‐UTR. Notably, the expression of these four miR‐BARTs represented more than 10% of all EBV‐encoded miRNAs in tumor cells, while downregulation of ATM expression was commonly detected in all of our tested sequenced samples. In addition, downregulation of ATM was also observed in primary NPC tissues in both qRT‐PCR (16 NP and 45 NPC cases) and immunohistochemical staining (35 NP and 46 NPC cases) analysis. Modulation of ATM expression by BART5‐5p, BART7‐3p, BART9‐3p, and BART14‐3p was demonstrated in the transient transfection assays. These findings suggest that EBV uses miRNA machinery as a key mechanism to control the ATM signaling pathway in NPC cells. By suppressing these endogenous miR‐BARTs in EBV‐positive NPC cells, we further demonstrated the novel function of miR‐BARTs in inhibiting Zta‐induced lytic reactivation. These findings imply that the four viral miRNAs work co‐operatively to modulate ATM activity in response to DNA damage and to maintain viral latency, contributing to the tumorigenesis of NPC.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) | |||||||||||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
|||||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Pharynx -- Cancer -- Research, Cancer -- Southeast Asia | |||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | The Journal of Pathology | |||||||||||||||||||||||||||||||||||||||
| Publisher: | Wiley | |||||||||||||||||||||||||||||||||||||||
| ISSN: | 0022-3417 | |||||||||||||||||||||||||||||||||||||||
| Official Date: | April 2018 | |||||||||||||||||||||||||||||||||||||||
| Dates: |
|
|||||||||||||||||||||||||||||||||||||||
| Volume: | 244 | |||||||||||||||||||||||||||||||||||||||
| Number: | 4 | |||||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 394-407 | |||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1002/path.5018 | |||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
