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EBV-encoded miRNAs target ATM-mediated response in nasopharyngeal carcinoma

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Lung, Raymond W-M., Hau, Pok-Man, Yu, Ken H-O., Yip, Kevin Y., Tong, Joanna H-M., Chak, Wing-Po, Chan, Anthony W-H., Lam, Ka-Hei, Lo, Angela Kwok-Fung, Tin, Edith K-Y., Chau, Shuk-Ling, Pang, Jesse C-S., Kwan, Johnny S-H., Busson, Pierre, Young, Lawrence S., Yap, Lee-Fah, Tsao, Sai-Wah, To, Ka-Fai and Lo, Kwok-Wai (2018) EBV-encoded miRNAs target ATM-mediated response in nasopharyngeal carcinoma. The Journal of Pathology, 244 (4). pp. 394-407. doi:10.1002/path.5018

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Official URL: http://dx.doi.org/10.1002/path.5018

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Abstract

Nasopharyngeal carcinoma (NPC) is a highly invasive epithelial malignancy that is prevalent in southern China and Southeast Asia. It is consistently associated with latent Epstein–Barr virus (EBV) infection. In NPC, miR‐BARTs, the EBV‐encoded miRNAs derived from BamH1‐A rightward transcripts, are abundantly expressed and contribute to cancer development by targeting various cellular and viral genes. In this study, we establish a comprehensive transcriptional profile of EBV‐encoded miRNAs in a panel of NPC patient‐derived xenografts and an EBV‐positive NPC cell line by small RNA sequencing. Among the 40 miR‐BARTs, predominant expression of 22 miRNAs was consistently detected in these tumors. Among the abundantly expressed EBV‐miRNAs, BART5‐5p, BART7‐3p, BART9‐3p, and BART14‐3p could negatively regulate the expression of a key DNA double‐strand break (DSB) repair gene, ataxia telangiectasia mutated (ATM), by binding to multiple sites on its 3'‐UTR. Notably, the expression of these four miR‐BARTs represented more than 10% of all EBV‐encoded miRNAs in tumor cells, while downregulation of ATM expression was commonly detected in all of our tested sequenced samples. In addition, downregulation of ATM was also observed in primary NPC tissues in both qRT‐PCR (16 NP and 45 NPC cases) and immunohistochemical staining (35 NP and 46 NPC cases) analysis. Modulation of ATM expression by BART5‐5p, BART7‐3p, BART9‐3p, and BART14‐3p was demonstrated in the transient transfection assays. These findings suggest that EBV uses miRNA machinery as a key mechanism to control the ATM signaling pathway in NPC cells. By suppressing these endogenous miR‐BARTs in EBV‐positive NPC cells, we further demonstrated the novel function of miR‐BARTs in inhibiting Zta‐induced lytic reactivation. These findings imply that the four viral miRNAs work co‐operatively to modulate ATM activity in response to DNA damage and to maintain viral latency, contributing to the tumorigenesis of NPC.

Item Type: Journal Article
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Pharynx -- Cancer -- Research, Cancer -- Southeast Asia
Journal or Publication Title: The Journal of Pathology
Publisher: Wiley
ISSN: 0022-3417
Official Date: April 2018
Dates:
DateEvent
April 2018Published
16 December 2017Available
5 December 2017Accepted
Date of first compliant deposit: 20 March 2019
Volume: 244
Number: 4
Page Range: pp. 394-407
DOI: 10.1002/path.5018
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
VCF2014017Chinese University of Hong Konghttp://dx.doi.org/10.13039/501100004853
471211Research Grants Council, University Grants Committeehttp://dx.doi.org/10.13039/501100002920
470312Research Grants Council, University Grants Committeehttp://dx.doi.org/10.13039/501100002920
471413Research Grants Council, University Grants Committeehttp://dx.doi.org/10.13039/501100002920
14104415 Research Grants Council, University Grants Committeehttp://dx.doi.org/10.13039/501100002920
14138016Research Grants Council, University Grants Committeehttp://dx.doi.org/10.13039/501100002920
CUHK8/CRF/11R Chinese University of Hong Konghttp://dx.doi.org/10.13039/501100004853
C7027-16GChinese University of Hong Konghttp://dx.doi.org/10.13039/501100004853
T12-401/13RChinese University of Hong Konghttp://dx.doi.org/10.13039/501100004853
FP013-2016Malaysia. Kementerian Pengajian Tinggihttp://viaf.org/viaf/149296907
IF016-2017Newton-Ungku Omar FundUNSPECIFIED
MR/P013201/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

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