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Purification, characterization and degradation performance of a novel dextranase from Penicillium cyclopium CICC-4022
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Huang, Ruijie, Zhong, Lei, Xie, Fengwei, Wei, Liming, Gan, Lanfang, Wang, Xuejiao and Liao, Anping (2019) Purification, characterization and degradation performance of a novel dextranase from Penicillium cyclopium CICC-4022. International Journal of Molecular Sciences, 20 (6). 1360. doi:10.3390/ijms20061360 ISSN 1422-0067.
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WRAP-purification-characterization-degradation-performance-novel-dextranase-Penicillium-cyclopium-Wei-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (758Kb) | Preview |
Official URL: http://dx.doi.org/10.3390/ijms20061360
Abstract
A novel dextranase was purified from Penicillium cyclopium CICC-4022 by ammonium sulfate fractional precipitation and gel filtration chromatography. The effects of temperature, pH and some metal ions and chemicals on dextranase activity were investigated. Subsequently, the dextranase was used to produce dextran with specific molecular mass. Weight-average molecular mass (Mw) and the ratio of weight-average molecular mass/number-average molecular mass, or polydispersity index (Mw/Mn), of dextran were measured by multiple-angle laser light scattering (MALS) combined with gel permeation chromatography (GPC). The dextranase was purified to 16.09-fold concentration; the recovery rate was 29.17%; and the specific activity reached 350.29 U/mg. Mw of the dextranase was 66 kDa, which is similar to dextranase obtained from other Penicillium species reported previously. The highest activity was observed at 55 °C and a pH of 5.0. This dextranase was identified as an endodextranase, which specifically degraded the α-1,6 glucosidic bonds of dextran. According to metal ion dependency tests, Li+, Na+ and Fe2+ were observed to effectively improve the enzymatic activity. In particular, Li+ could improve the activity to 116.28%. Furthermore, the dextranase was efficient at degrading dextran and the degradation rate can be well controlled by the dextranase activity, substrate concentration and reaction time. Thus, our results demonstrate the high potential of this dextranase from Penicillium cyclopium CICC-4022 as an efficient enzyme to produce specific clinical dextrans.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QK Botany Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | |||||||||
| Library of Congress Subject Headings (LCSH): | Dextranase, Penicillium | |||||||||
| Journal or Publication Title: | International Journal of Molecular Sciences | |||||||||
| Publisher: | M D P I AG | |||||||||
| ISSN: | 1422-0067 | |||||||||
| Official Date: | 18 March 2019 | |||||||||
| Dates: |
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| Volume: | 20 | |||||||||
| Number: | 6 | |||||||||
| Article Number: | 1360 | |||||||||
| DOI: | 10.3390/ijms20061360 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 21 March 2019 | |||||||||
| Date of first compliant Open Access: | 27 March 2019 | |||||||||
| RIOXX Funder/Project Grant: |
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