The antigenicity of the chicken anaemia virus protein VP3 (Apoptin)
UNSPECIFIED. (2001) The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). AVIAN PATHOLOGY, 30 (6). pp. 613-619. ISSN 0307-9457Full text not available from this repository.
Chicken anaemia virus protein VP3 (Apoptin) was cloned and expressed as a recombinant protein and evaluated for its suitability as a serodiagnostic reagent. VP3 was expressed as a fusion protein either with glutathione S-transferase or with a six-histidine tag. Both recombinant proteins reacted specifically with anti-VP3 monoclonal antibodies and with serum from vaccinated chickens by Western blot and by enzyme-linked immunosorbent assay (ELISA). However, when testing sera from birds of different ages and genetic backgrounds, high non-specific reactions were evident and false positives were observed, especially in older birds. This suggests that VP3 is poorly immunogenic during infection and low antibody concentrations are masked by non-specific reactions. Thus, VP3 is not suitable for use as antigen in ELISAs.
|Item Type:||Journal Article|
|Subjects:||S Agriculture > SF Animal culture|
|Journal or Publication Title:||AVIAN PATHOLOGY|
|Official Date:||December 2001|
|Number of Pages:||7|
|Page Range:||pp. 613-619|
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