UNSPECIFIED (2001) The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). AVIAN PATHOLOGY, 30 (6). pp. 613-619. ISSN 0307-9457

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Abstract

Chicken anaemia virus protein VP3 (Apoptin) was cloned and expressed as a recombinant protein and evaluated for its suitability as a serodiagnostic reagent. VP3 was expressed as a fusion protein either with glutathione S-transferase or with a six-histidine tag. Both recombinant proteins reacted specifically with anti-VP3 monoclonal antibodies and with serum from vaccinated chickens by Western blot and by enzyme-linked immunosorbent assay (ELISA). However, when testing sera from birds of different ages and genetic backgrounds, high non-specific reactions were evident and false positives were observed, especially in older birds. This suggests that VP3 is poorly immunogenic during infection and low antibody concentrations are masked by non-specific reactions. Thus, VP3 is not suitable for use as antigen in ELISAs.

Item Type:	Journal Article
Subjects:	S Agriculture > SF Animal culture
Journal or Publication Title:	AVIAN PATHOLOGY
Publisher:	CARFAX PUBLISHING
ISSN:	0307-9457
Date:	December 2001
Volume:	30
Number:	6
Number of Pages:	7
Page Range:	pp. 613-619
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/11541

Data sourced from Thomson Reuters' Web of Knowledge

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