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The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci
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Singh, Anand K., Choudhury, Subhendu Roy, De, Sandip, Zhang, Jie, Kissane, Stephen, Dwivedi, Vibha, Ramanathan, Preethi, Petric, Marija, Orsini, Luisa, Hebenstreit, Daniel and Brogna, Saverio (2019) The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci. eLife, 8 . e41444. doi:10.7554/eLife.41444 ISSN 2050-084X.
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WRAP-RNA-helicase-UPF1-associates-mRNAs-co-transcriptionally-Zhang-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (10Mb) | Preview |
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WRAP-RNA-helicase-UPF1-associates-mRNAs-co-transcriptionally-required-release-mRNAs-gene-loci-Hebenstreit-2019.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (90Mb) |
Official URL: http://dx.doi.org/10.7554/eLife.41444
Abstract
UPF1 is an RNA helicase that is required for efficient nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3'UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and mRNA export from the nucleus.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Messenger RNA, RNA | ||||||||||||||||||
Journal or Publication Title: | eLife | ||||||||||||||||||
Publisher: | eLife Sciences Publications Ltd. | ||||||||||||||||||
ISSN: | 2050-084X | ||||||||||||||||||
Official Date: | 25 March 2019 | ||||||||||||||||||
Dates: |
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Volume: | 8 | ||||||||||||||||||
Article Number: | e41444 | ||||||||||||||||||
DOI: | 10.7554/eLife.41444 | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 25 March 2019 | ||||||||||||||||||
Date of first compliant Open Access: | 28 March 2019 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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