Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Synchrotron XRF imaging of Alzheimer’s disease basal ganglia reveals linear dependence of high-field magnetic resonance microscopy on tissue iron concentration

Tools
- Tools
+ Tools

Finnegan, Mary E., Visanji, Naomi P., Romero-Canelon, Isolda, House, Emily, Rajan, Surya, Mosselmans, Frederick W., Hazrati, Lili-Naz, Dobson, Jon and Collingwood, Joanna F. (2019) Synchrotron XRF imaging of Alzheimer’s disease basal ganglia reveals linear dependence of high-field magnetic resonance microscopy on tissue iron concentration. Journal of Neuroscience Methods, 319 . pp. 28-39. doi:10.1016/j.jneumeth.2019.03.002 ISSN 0165-0270.

[img]
Preview
PDF
WRAP-synchrotron-XRF-imaging-Alzheimer’s-disease-basal-ganglia-Collingwood-2019.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (2236Kb) | Preview
Official URL: http://dx.doi.org/10.1016/j.jneumeth.2019.03.002

Request Changes to record.

Abstract

Background

Chemical imaging of the human brain has great potential for diagnostic and monitoring purposes. The heterogeneity of human brain iron distribution, and alterations to this distribution in Alzheimer’s disease, indicate iron as a potential endogenous marker. The influence of iron on certain magnetic resonance imaging (MRI) parameters increases with magnetic field, but is under-explored in human brain tissues above 7 T.

New Method

Magnetic resonance microscopy at 9.4 T is used to calculate parametric images of chemically-unfixed post-mortem tissue from Alzheimer’s cases (n = 3) and healthy controls (n = 2). Iron-rich regions including caudate nucleus, putamen, globus pallidus and substantia nigra are analysed prior to imaging of total iron distribution with synchrotron X-ray fluorescence mapping. Iron fluorescence calibration is achieved with adjacent tissue blocks, analysed by inductively coupled plasma mass spectrometry or graphite furnace atomic absorption spectroscopy.

Results

Correlated MR images and fluorescence maps indicate linear dependence of R2, R2* and R2’ on iron at 9.4 T, for both disease and control, as follows: [R2(s−1) = 0.072[Fe] + 20]; [R2*(s−1) = 0.34[Fe] + 37]; [R2’(s−1) = 0.26[Fe] + 16] for Fe in μg/g tissue (wet weight).

Comparison with Existing Methods

This method permits simultaneous non-destructive imaging of most bioavailable elements. Iron is the focus of the present study as it offers strong scope for clinical evaluation; the approach may be used more widely to evaluate the impact of chemical elements on clinical imaging parameters.

Conclusion

The results at 9.4 T are in excellent quantitative agreement with predictions from experiments performed at lower magnetic fields.

Item Type: Journal Article
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Faculty of Science, Engineering and Medicine > Engineering > Engineering
Library of Congress Subject Headings (LCSH): Alzheimer's disease -- Research
Journal or Publication Title: Journal of Neuroscience Methods
Publisher: Elsevier BV
ISSN: 0165-0270
Official Date: 1 May 2019
Dates:
DateEvent
1 May 2019Published
6 March 2019Available
2 March 2019Accepted
Volume: 319
Page Range: pp. 28-39
DOI: 10.1016/j.jneumeth.2019.03.002
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 28 March 2019
Date of first compliant Open Access: 28 March 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDAlzheimer's Societyhttp://dx.doi.org/10.13039/501100000320
EP/K035193/1 [EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
EP/M028186/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
SP1125 Diamond Light Sourcehttp://dx.doi.org/10.13039/100011889
SP7453Diamond Light Sourcehttp://dx.doi.org/10.13039/100011889
DMR-1157490National Science Foundationhttp://dx.doi.org/10.13039/100000001
UNSPECIFIEDFlorida. Department of StateUNSPECIFIED
Translational Medicine: Experimental Medicine Networkof ExcellenceBirmingham Science CityUNSPECIFIED
UNSPECIFIEDAdvantage West Midlands (AWM)UNSPECIFIED

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us