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Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction
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Cheffings, Thomas H., Burroughs, Nigel John and Balasubramanian, Mohan K. (2019) Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction. Molecular Biology of the Cell, 30 (8). pp. 933-1049. doi:10.1091/mbc.E18-08-0511 ISSN 1059-1524.
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WRAP-actin-turnover-ensures-uniform-tension-distribution-cytokinetic-actomyosin-ring-contraction-Balasubramanian-2019.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (4Mb) |
Official URL: http://dx.doi.org/10.1091/mbc.E18-08-0511
Abstract
In many eukaryotes, cytokinesis is facilitated by the contraction of an actomyosin ring (AMR). The exact mechanisms that lead to this contractility are unknown, although some models posit that actin turnover in the AMR is essential. The effect of reduced actin dynamics during AMR formation has been well studied in Schizosaccharomyces pombe; however, the corresponding effects on AMR contraction are not well understood. By using mutants of the fission yeast actin severing protein Adf1, we observed that contracting AMRs display a “peeling” phenotype, where bundles of actin and myosin peel off from one side of the AMR, and are pulled across to the opposite side. This occurs multiple times during cytokinesis and is dependent on the activity of myosins Myo2, Myp2, and Myo51. We found that the distribution of Myo2 in the AMR anticorrelates with the location of peeling events, suggesting that peeling is caused by a nonuniform tension distribution around the AMR, and that one of the roles of actin turnover is to maintain a uniform tension distribution around the AMR.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Science > Mathematics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Molecular Biology of the Cell | ||||||
Publisher: | American Society for Cell Biology | ||||||
ISSN: | 1059-1524 | ||||||
Official Date: | 1 April 2019 | ||||||
Dates: |
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Volume: | 30 | ||||||
Number: | 8 | ||||||
Page Range: | pp. 933-1049 | ||||||
DOI: | 10.1091/mbc.E18-08-0511 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 11 April 2019 | ||||||
Date of first compliant Open Access: | 15 August 2019 |
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