UNSPECIFIED (2001) Macromolecular crowding: obvious but underappreciated. [Journal Item]

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Abstract

Biological macromolecules evolve and function within intracellular environments that are crowded with other macromolecules. Crowding results in surprisingly large quantitative effects on both the rates and the equilibria of interactions involving macromolecules, but such interactions are commonly studied outside the cell in uncrowded buffers. The addition of high concentrations of natural and synthetic macromolecules to such buffers enables crowding to be mimicked in vitro, and should be encouraged as a routine variable to study. The stimulation of protein aggregation by crowding might account for the existence of molecular chaperones that combat this effect. Positive results of crowding include enhancing the collapse of polypeptide chains into functional proteins, the assembly of oligomeric structures and the efficiency of action of some molecular chaperones and metabolic pathways.

Item Type:	Journal Item
Subjects:	Q Science > QD Chemistry
Journal or Publication Title:	TRENDS IN BIOCHEMICAL SCIENCES
Publisher:	ELSEVIER SCIENCE LONDON
ISSN:	0968-0004
Date:	October 2001
Volume:	26
Number:	10
Number of Pages:	8
Page Range:	pp. 597-604
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/11627

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