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Changes in nanoscale chain assembly in sweet potato starch lamellae by downregulation of biosynthesis enzymes
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Zhang, Binjia, Zhou, Wenzhi, Qiao, Dongling, Zhang, Peng, Zhao, Siming, Zhang, Liang and Xie, Fengwei (2019) Changes in nanoscale chain assembly in sweet potato starch lamellae by downregulation of biosynthesis enzymes. Journal of Agricultural and Food Chemistry, 67 (22). pp. 6302-6312. doi:10.1021/acs.jafc.8b06523
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WRAP-Changes-nanoscale-sweet-potato-starch-enzymes-Xie-2019.pdf - Accepted Version - Requires a PDF viewer. Download (2266Kb) | Preview |
Official URL: https://doi.org/10.1021/acs.jafc.8b06523
Abstract
Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QP Physiology T Technology > TP Chemical technology |
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| Divisions: | Other > Institute of Advanced Study Faculty of Science > WMG (Formerly the Warwick Manufacturing Group) |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Starch, Biosynthesis, Sweet potatoes, Enzymes | |||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Journal of Agricultural and Food Chemistry | |||||||||||||||||||||||||||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||||||||||||||||||||||||||
| ISSN: | 1520-5118 | |||||||||||||||||||||||||||||||||
| Official Date: | 5 June 2019 | |||||||||||||||||||||||||||||||||
| Dates: |
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| Date of first compliant deposit: | 6 June 2019 | |||||||||||||||||||||||||||||||||
| Volume: | 67 | |||||||||||||||||||||||||||||||||
| Number: | 22 | |||||||||||||||||||||||||||||||||
| Page Range: | pp. 6302-6312 | |||||||||||||||||||||||||||||||||
| DOI: | 10.1021/acs.jafc.8b06523 | |||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||
| Publisher Statement: | “This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].” | |||||||||||||||||||||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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