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Changes in nanoscale chain assembly in sweet potato starch lamellae by downregulation of biosynthesis enzymes

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Zhang, Binjia, Zhou, Wenzhi, Qiao, Dongling, Zhang, Peng, Zhao, Siming, Zhang, Liang and Xie, Fengwei (2019) Changes in nanoscale chain assembly in sweet potato starch lamellae by downregulation of biosynthesis enzymes. Journal of Agricultural and Food Chemistry, 67 (22). pp. 6302-6312. doi:10.1021/acs.jafc.8b06523

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Official URL: https://doi.org/10.1021/acs.jafc.8b06523

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Abstract

Granule-bound starch synthase I (GBSSI) and starch-branching enzymes I and II (SBEI and SBEII) are crucial enzymes that biosynthesize starches with varied apparent amylose contents and amylopectin branching structure. With a sweet potato (Ipomoea batatas [L.] Lam. cv. Xushu22), this work shows that downregulating GBSSI (for waxy starch) or SBE (for high-amylose starch) activity allowed the formation of new semicrystalline lamellae (named Type II) in sweet potato starch in addition to the widely reported Type I lamellae. Small-angle X-ray scattering (SAXS) results show that, compared with Type I lamellae, Type II lamellae displayed increased average thickness and thickness-distribution width, with thickened amorphous and crystalline components. The size-exclusion-chromatography (SEC) data revealed mainly two enzyme sets, (i) and (ii), synthesizing amylopectin chains. Reducing the GBSSI or SBE activity increased the amounts of amylopectin long chains (degree of polymerization (DP) ≥ 33). Combined SAXS and SEC analyses indicate that parts of these long chains from enzyme set (i) could be confined to Type II lamellae, followed by DP ≤ 32 short chains in Type I lamellae and the rest of the long chains from enzyme sets (i) and (ii) spanning more than a single lamella.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
T Technology > TP Chemical technology
Divisions: Other > Institute of Advanced Study
Faculty of Science > WMG (Formerly the Warwick Manufacturing Group)
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Starch, Biosynthesis, Sweet potatoes, Enzymes
Journal or Publication Title: Journal of Agricultural and Food Chemistry
Publisher: American Chemical Society (ACS)
ISSN: 1520-5118
Official Date: 5 June 2019
Dates:
DateEvent
5 June 2019Published
10 April 2019Available
29 March 2019Accepted
Date of first compliant deposit: 6 June 2019
Volume: 67
Number: 22
Page Range: pp. 6302-6312
DOI: 10.1021/acs.jafc.8b06523
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: “This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].”
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
31701637 [NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
31801582[NSFC] National Natural Science Foundation of Chinahttp://dx.doi.org/10.13039/501100001809
2662016QD008[MEPRC] Ministry of Education of the People's Republic of Chinahttp://dx.doi.org/10.13039/501100002338
XDPB04[CAS] Chinese Academy of Scienceshttp://dx.doi.org/10.13039/501100002367
2018M642865China Postdoctoral Science Foundationhttp://dx.doi.org/10.13039/501100002858
UNSPECIFIEDChina Association for Science and Technologyhttp://dx.doi.org/10.13039/100010097
UNSPECIFIEDChutian Sholars Program of Hubei ProvinceUNSPECIFIED
Shishan Sholars ProgramHuazhong Agricultural Universityhttp://dx.doi.org/10.13039/501100007925
UNSPECIFIEDFP7 People: Marie-Curie Actionshttp://dx.doi.org/10.13039/100011264
UNSPECIFIEDUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741

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