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Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement

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UNSPECIFIED (2001) Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement. JOURNAL OF VIROLOGICAL METHODS, 98 (2). pp. 167-169.

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Abstract

Improving the virus particle: infectious unit ratio is a continuing goal for animal virologists. It is demonstrated for an influenza A virus that decreasing the size of the inoculum volume to 10 mul per well of a 96-well plate was as effective as using centrifugal force with inoculum up to 250 mul. Both achieved a 7.5-fold increase in infectivity in monolayers of MDCK cells compared with standard conditions. The underlying principle of both methods is to bring virus particles into close contact with cell receptors. (C) 2001 Elsevier Science B.V. All rights reserved.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
T Technology > TP Chemical technology
Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title: JOURNAL OF VIROLOGICAL METHODS
Publisher: ELSEVIER SCIENCE BV
ISSN: 0166-0934
Official Date: November 2001
Dates:
DateEvent
November 2001UNSPECIFIED
Volume: 98
Number: 2
Number of Pages: 3
Page Range: pp. 167-169
Publication Status: Published

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