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Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement
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UNSPECIFIED (2001) Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement. JOURNAL OF VIROLOGICAL METHODS, 98 (2). pp. 167-169.
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Abstract
Improving the virus particle: infectious unit ratio is a continuing goal for animal virologists. It is demonstrated for an influenza A virus that decreasing the size of the inoculum volume to 10 mul per well of a 96-well plate was as effective as using centrifugal force with inoculum up to 250 mul. Both achieved a 7.5-fold increase in infectivity in monolayers of MDCK cells compared with standard conditions. The underlying principle of both methods is to bring virus particles into close contact with cell receptors. (C) 2001 Elsevier Science B.V. All rights reserved.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology Q Science > QR Microbiology > QR355 Virology |
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| Journal or Publication Title: | JOURNAL OF VIROLOGICAL METHODS | ||||
| Publisher: | ELSEVIER SCIENCE BV | ||||
| ISSN: | 0166-0934 | ||||
| Official Date: | November 2001 | ||||
| Dates: |
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| Volume: | 98 | ||||
| Number: | 2 | ||||
| Number of Pages: | 3 | ||||
| Page Range: | pp. 167-169 | ||||
| Publication Status: | Published |
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