Sand, Charles, Njunge, James, Mwongeli, Joyce, Mutunga, Martin, Chege, Timothy, Gicheru, Elijah, Gardiner, Elizabeth, Gwela, Agnes, Drysdale, Simon, Green, Christopher, Berkley, James, Nokes, D. James and Pollard, Andrew J. (2019) Airway response to respiratory syncytial virus has incidental antibacterial effects. Nature Communications, 10 (1). 2218. doi:10.1038/s41467-019-10222-z

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Abstract

RSV infection is typically associated with secondary bacterial infection. We hypothesise that the local airway immune response to RSV has incidental antibacterial effects. Using coordinated proteomics and metagenomics analysis we simultaneously analysed the microbiota and proteomes of the upper airway and determined direct antibacterial activity in airway secretions of RSV-infected children. Here, we report that the airway abundance of Streptococcus was higher in samples collected at the time of RSV infection compared with samples collected one month later. RSV infection is associated with neutrophil influx into the airway and degranulation and is marked by overexpression of proteins with known antibacterial activity including BPI, EPX, MPO and AZU1. Airway secretions of children infected with RSV, have significantly greater antibacterial activity compared to RSV-negative controls. This RSV-associated, neutrophil-mediated antibacterial response in the airway appears to act as a regulatory mechanism that modulates bacterial growth in the airways of RSV-infected children.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology R Medicine > RJ Pediatrics
Divisions:	Faculty of Science > Life Sciences (2010- ) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Respiratory syncytial virus, Bacterial diseases in children, Respiratory organs -- Obstructions -- Treatment
Journal or Publication Title:	Nature Communications
Publisher:	Nature Publishing Group
ISSN:	2041-1723
Official Date:	17 May 2019
Dates:	Date Event 17 May 2019 Published 26 April 2019 Accepted
Date of first compliant deposit:	7 May 2019
Volume:	10
Number:	1
Article Number:	2218
DOI:	10.1038/s41467-019-10222-z
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID WT105882MA Wellcome Trust http://dx.doi.org/10.13039/100010269
Related URLs:	Publisher

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