Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport

Tools
- Tools
+ Tools

Siddiqui, Nida, Zwetsloot, Alexander J., Bachmann, Alice, Roth, Daniel, Hussain, Hamdi, Brandt, Jonathan, Kaverina, Irina and Straube, Anne (2019) PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Nature Communications, 10 . 2693 . doi:10.1038/s41467-019-10644-9 ISSN 2041-1723.

[img]
Preview
PDF
WRAP-PTPN21-Hook3-relieve-activate-transport-Straube-2019.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (6Mb) | Preview
Official URL: https://doi.org/10.1038/s41467-019-10644-9

Request Changes to record.

Abstract

The kinesin-3 KIF1C is a fast organelle transporter implicated in the transport of dense core vesicles in neurons and the delivery of integrins to cell adhesions. Here we report the mechanisms of autoinhibition and release that control the activity of KIF1C. We show that the microtubule binding surface of KIF1C motor domain interacts with its stalk and that these autoinhibitory interactions are released upon binding of protein tyrosine phosphatase PTPN21. The FERM domain of PTPN21 stimulates dense core vesicle transport in primary hippocampal neurons and rescues integrin trafficking in KIF1C-depleted cells. In vitro, human full-length KIF1C is a processive, plus-end directed motor. Its landing rate onto microtubules increases in the presence of either PTPN21 FERM domain or the cargo adapter Hook3 that binds the same region of KIF1C tail. This autoinhibition release mechanism allows cargo-activated transport and might enable motors to participate in bidirectional cargo transport without undertaking a tug-of-war.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Kinesin, Kinesin -- Movements, Cell organelles, Integrins, Cell adhesion, Microtubules
Journal or Publication Title: Nature Communications
Publisher: Nature Publishing Group
ISSN: 2041-1723
Official Date: 19 June 2019
Dates:
DateEvent
19 June 2019Published
23 May 2019Accepted
Volume: 10
Article Number: 2693
DOI: 10.1038/s41467-019-10644-9
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 10 July 2019
Date of first compliant Open Access: 17 July 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDLister Institute of Preventive Medicinehttp://dx.doi.org/10.13039/501100001255
200870/Z/16/ZWellcome Trusthttp://dx.doi.org/10.13039/100010269
S/13/42/30377British Heart Foundationhttp://dx.doi.org/10.13039/501100000274
Summer StudentshipLister Institute of Preventive Medicinehttp://dx.doi.org/10.13039/501100001255
Chancellor’s International PhD ScholarshipUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
EP/F500378/1, MOAC-CTD[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
MR/N014294/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
WMS ScholarshipUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
Open Access Version:
  • Publisher

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us