LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system
UNSPECIFIED. (2001) LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system. JOURNAL OF CLINICAL MICROBIOLOGY, 39 (9). pp. 3056-3059. ISSN 0095-1137Full text not available from this repository.
A conventional multiplex PCR assay that detects herpes simplex virus type 1 (HSV-1), HSV-2, varicella-zoster virus, and enteroviruses for the diagnosis of central nervous system infections was modified to be performed using the LightCycler system. The sensitivity of detection of each of the viruses using the LightCycler assay was compared to that of the conventional assay using external quality assessment material. The assays had equivalent sensitivities, but the LightCycler assay was more rapid, reduced the risk of contamination, and used an amplicon detection format that demonstrated greater discrimination than a get electrophoresis method.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||JOURNAL OF CLINICAL MICROBIOLOGY|
|Publisher:||AMER SOC MICROBIOLOGY|
|Number of Pages:||4|
|Page Range:||pp. 3056-3059|
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