Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Expanding the zebrafish genetic code through site-specific introduction of azido-lysine, bicyclononyne-lysine, and diazirine-lysine

Tools
- Tools
+ Tools

Syed, Junetha, Palani, Saravanan, Clarke, Scott T., Asad, Zainab, Bottrill, Andrew R., Jones, Alexandra M.E., Sampath, Karuna and Balasubramanian, Mohan K. (2019) Expanding the zebrafish genetic code through site-specific introduction of azido-lysine, bicyclononyne-lysine, and diazirine-lysine. International Journal of Molecular Sciences, 20 (10). 2577. doi:10.3390/ijms20102577 ISSN 1422-0067.

[img]
Preview
PDF
WRAP-expanding-zebrafish-genetic-code-Jones-2019.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (2620Kb) | Preview
Official URL: http://dx.doi.org/10.3390/ijms20102577

Request Changes to record.

Abstract

Site-specific incorporation of un-natural amino acids (UNAA) is a powerful approach to engineer and understand protein function. Site-specific incorporation of UNAAs is achieved through repurposing the amber codon (UAG) as a sense codon for the UNAA, using a tRNACUA that base pairs with an UAG codon in the mRNA and an orthogonal amino-acyl tRNA synthetase (aaRS) that charges the tRNACUA with the UNAA. Here, we report an expansion of the zebrafish genetic code to incorporate the UNAAs, azido-lysine (AzK), bicyclononyne-lysine (BCNK), and diazirine-lysine (AbK) into green fluorescent protein (GFP) and glutathione-s-transferase (GST). We also present proteomic evidence for UNAA incorporation into GFP. Our work sets the stage for the use of AzK, BCNK, and AbK introduction into proteins as a means to investigate and engineer their function in zebrafish.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Journal or Publication Title: International Journal of Molecular Sciences
Publisher: M D P I AG
ISSN: 1422-0067
Official Date: 26 May 2019
Dates:
DateEvent
26 May 2019Published
23 May 2019Accepted
Volume: 20
Number: 10
Article Number: 2577
DOI: 10.3390/ijms20102577
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 26 June 2019
Date of first compliant Open Access: 26 June 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
WT101885MAWellcome Trusthttp://dx.doi.org/10.13039/100010269
WM130042[RS] Royal Societyhttp://dx.doi.org/10.13039/501100000288
671083H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
EP/F500378/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us