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Tissue distribution and specific contribution of Arabidopsis FAD7 and FAD8 plastid desaturases to the JA- and ABA-mediated cold stress or defense responses
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Soria-García, Ángel, Rubio, Maria C., Lagunas, Beatriz, López-Gomollón, Sara, Lujan, María de los Ángeles , Díaz-Guerra, Raúl , Picorel, Rafael and Alfonso, Miguel (2019) Tissue distribution and specific contribution of Arabidopsis FAD7 and FAD8 plastid desaturases to the JA- and ABA-mediated cold stress or defense responses. Plant and Cell Physiology, 60 (5). pp. 1025-1040. doi:10.1093/pcp/pcz017
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WRAP-tissue-distribution-specific-contribution-Arabidopsis-Lagunas-2019.pdf - Accepted Version - Requires a PDF viewer. Download (766Kb) | Preview |
Official URL: http://dx.doi.org/10.1093/pcp/pcz017
Abstract
To overcome the difficulties to analyze membrane desaturases at the protein level, transgenic Arabidopsis plants expressing the plastidial AtFAD7 and AtFAD8 ω-3 desaturases fused to green fluorescent protein, under the control of their endogenous promoters, were generated and their tissue relative abundance was studied. Gene expression, glucuronidase promoter activity, immunoblot and confocal microscopy analyses indicated that AtFAD7 is the major ω-3 desaturase in leaves when compared to AtFAD8. This higher abundance of AtFAD7 was consistent with its higher promoter activity and could be related with its specificity for the abundant leaf galactolipids. AtFAD7 was also present in roots but at much lower level than leaves. AtFAD8 expression and protein abundance in leaves was consistent with its lower promoter activity, suggesting that transcriptional control modulates the abundance of both desaturases in leaves. AtFAD7 protein levels increased in response to wounding but not to jasmonate (JA), and decreased upon abscisic acid (ABA) treatment. Conversely, AtFAD8 protein levels increased upon cold or JA exposure and decreased at high temperatures, but did not respond to ABA or wounding. These results indicated specific and non-redundant roles for the plastidial ω-3 desaturases in defense, temperature stress or phytohormone mediated responses and a tight coordination of their activities between biotic and abiotic stress signaling pathways. Our data suggested that transcriptional regulation was crucial for this coordination. Finally, bimolecular fluorescence complementation analysis showed that both AtFAD7 and AtFAD8 interact with the AtFAD6 ω-6 desaturase in vivo, suggesting that quaternary complexes are involved in trienoic fatty acid production within the plastid membranes.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QH Natural history S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Crops -- Effect of stress on, Crops -- Physiology, Transgenic plants , Gene expression, Plant hormones | ||||||||||||
| Journal or Publication Title: | Plant and Cell Physiology | ||||||||||||
| Publisher: | Oxford Academic | ||||||||||||
| ISSN: | 0032-0781 | ||||||||||||
| Official Date: | May 2019 | ||||||||||||
| Dates: |
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| Volume: | 60 | ||||||||||||
| Number: | 5 | ||||||||||||
| Page Range: | pp. 1025-1040 | ||||||||||||
| DOI: | 10.1093/pcp/pcz017 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Publisher Statement: | This is a pre-copyedited, author-produced version of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record Ángel Soria-García, María C Rubio, Beatriz Lagunas, Sara López-Gomollón, María de los Ángeles Luján, Raúl Díaz-Guerra, Rafael Picorel, Miguel Alfonso, Tissue Distribution and Specific Contribution of Arabidopsis FAD7 and FAD8 Plastid Desaturases to the JA- and ABA-Mediated Cold Stress or Defense Responses, Plant and Cell Physiology, Volume 60, Issue 5, May 2019, Pages 1025–1040, https://doi.org/10.1093/pcp/pcz017 is available online at: https://doi.org/10.1093/pcp/pcz017 | ||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
| RIOXX Funder/Project Grant: |
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