UNSPECIFIED. (2001) Human granulosa-lutein cells express functional EP1 and EP2 prostaglandin receptors. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 285 (5). pp. 1089-1094. ISSN 0006-291X

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Abstract

Prostaglandin E-2 (PGE(2)) exerts mainly luteotrophic effects in the corpus luteum. In other tissues, PGE,, acts via specific PGE2 receptor subtypes including EP1, which modulates intracellular calcium ([Ca2+](i)) and EP2, which is coupled to cyclic AMP (cAMP) generation. We have therefore investigated the presence of functional EP1 and EP2 receptors using human granulosa-lutein (GL) cells. Reverse-transcription PCR revealed that GL cells expressed mRNA transcripts encoding both EP1 and EP2 receptors. When GL cells were challenged with ligands that can bind to both receptor subtypes (PGE2 and 16,16 dimethyl PGE) or exclusively to EP2 (butaprost), both cAMP formation and progesterone synthesis were stimulated. Furthermore, the cAMP response to these agonists could be significantly blocked by an EP1/2 antagonist AH6809 but not by an EP1-selective antagonist SC19220. Exposure of GL cells to 16,16-dm PGE(2) transiently raised [Ca2+](i) levels, which could be prevented by both AH6809 and SC19220. We therefore conclude that human GL cells express functional EP1 and EP2 receptors. (C) 2001 Academic Press.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Publisher:	ACADEMIC PRESS INC
ISSN:	0006-291X
Date:	3 August 2001
Volume:	285
Number:	5
Number of Pages:	6
Page Range:	pp. 1089-1094
Identification Number:	10.1006/bbrc.2001.5301
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/11894

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