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Isolation and flow cytometric analysis of immune cells from the ischemic mouse brain

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Pösel, Claudia, Möller, Karoline, Boltze, Johannes, Wagner, Daniel-Christoph and Weise, Gesa (2016) Isolation and flow cytometric analysis of immune cells from the ischemic mouse brain. Journal of Visualized Experiments (108). e53658. doi:10.3791/53658 ISSN 1940-087X.

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Official URL: http://dx.doi.org/10.3791/53658

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Abstract

Ischemic stroke initiates a robust inflammatory response that starts in the intravascular compartment and involves rapid activation of brain resident cells. A key mechanism of this inflammatory response is the migration of circulating immune cells to the ischemic brain facilitated by chemokine release and increased endothelial adhesion molecule expression. Brain-invading leukocytes are well-known contributing to early-stage secondary ischemic injury, but their significance for the termination of inflammation and later brain repair has only recently been noticed.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Journal or Publication Title: Journal of Visualized Experiments
Publisher: JoVE
ISSN: 1940-087X
Official Date: 2016
Dates:
DateEvent
2016Published
Number: 108
Article Number: e53658
DOI: 10.3791/53658
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)

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