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Isolation and flow cytometric analysis of immune cells from the ischemic mouse brain
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Pösel, Claudia, Möller, Karoline, Boltze, Johannes, Wagner, Daniel-Christoph and Weise, Gesa (2016) Isolation and flow cytometric analysis of immune cells from the ischemic mouse brain. Journal of Visualized Experiments (108). e53658. doi:10.3791/53658 ISSN 1940-087X.
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Official URL: http://dx.doi.org/10.3791/53658
Abstract
Ischemic stroke initiates a robust inflammatory response that starts in the intravascular compartment and involves rapid activation of brain resident cells. A key mechanism of this inflammatory response is the migration of circulating immune cells to the ischemic brain facilitated by chemokine release and increased endothelial adhesion molecule expression. Brain-invading leukocytes are well-known contributing to early-stage secondary ischemic injury, but their significance for the termination of inflammation and later brain repair has only recently been noticed.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Journal of Visualized Experiments | ||||
Publisher: | JoVE | ||||
ISSN: | 1940-087X | ||||
Official Date: | 2016 | ||||
Dates: |
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Number: | 108 | ||||
Article Number: | e53658 | ||||
DOI: | 10.3791/53658 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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