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Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR imaging at 3T : determination of the detection limit
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Fraidenraich, Diego, Lobsien, Donald, Dreyer, Antje Y., Stroh, Albrecht, Boltze, Johannes and Hoffmann, Karl-Titus (2013) Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR imaging at 3T : determination of the detection limit. PLoS One, 8 (5). e62644. doi:10.1371/journal.pone.0062644 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0062644
Abstract
Objectives
This study aimed to evaluate the detectability of stem cells labeled with very small iron oxide particles (VSOP) at 3T with susceptibility weighted (SWI) and T2* weighted imaging as a methodological basis for subsequent examinations in a large animal stroke model (sheep).
Materials and Methods
We examined ovine mesenchymal stem cells labeled with VSOP in agarose layer phantoms. The experiments were performed in 2 different groups, with quantities of 0–100,000 labeled cells per layer. 15 different SWI- and T2*-weighted sequences and 3 RF coils were used. All measurements were carried out on a clinical 3T MRI. Images of Group A were analyzed by four radiologists blinded for the number of cells, and rated for detectability according to a four-step scale. Images of Group B were subject to a ROI-based analysis of signal intensities. Signal deviations of more than the 0.95 confidence interval in cell containing layers as compared to the mean of the signal intensity of non cell bearing layers were considered significant.
Results
Group A: 500 or more labeled cells were judged as confidently visible when examined with a SWI-sequence with 0.15 mm slice thickness. Group B: 500 or more labeled cells showed a significant signal reduction in SWI sequences with a slice thickness of 0.25 mm. Slice thickness and cell number per layer had a significant influence on the amount of detected signal reduction.
Conclusion
500 VSOP labeled stem cells could be detected with SWI imaging at 3 Tesla using an experimental design suitable for large animal models.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | PLoS One | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1932-6203 | ||||
| Official Date: | 8 May 2013 | ||||
| Dates: |
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| Volume: | 8 | ||||
| Number: | 5 | ||||
| Article Number: | e62644 | ||||
| DOI: | 10.1371/journal.pone.0062644 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) |
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