The Library
Isolation of inflammatory cells from rat brain tissue after stroke
Tools
Tools
Tools
Möller, Karoline, Stahl, Tobias, Boltze, Johannes and Wagner, Daniel-Christoph (2012) Isolation of inflammatory cells from rat brain tissue after stroke. Experimental & Translational Stroke Medicine, 4 (1). p. 20. doi:10.1186/2040-7378-4-20 ISSN 2040-7378.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1186/2040-7378-4-20
Abstract
The pathophysiology of sterile inflammation following focal ischemic stroke is complex and not fully understood, but there is growing evidence that it offers several therapeutic options beyond the hitherto existing treatment strategies. The identification and quantification of infiltrating inflammatory cells in animal models of stroke is crucial both for understanding post-stroke inflammation and for drug target identification. Multicolor flow cytometry plays an important role in determining subtypes and quantity of leukocytes that infiltrate the brain tissue after stroke. Until now, most investigations have been performed in mice, most likely due to a significantly broader spectrum of disposable antibodies and available knockout models. Here, we introduce a specific and reproducible method to isolate leukocytes from rat brain specimen in the context of brain ischemia to ultimately allow multi-dimensional flow cytometric characterization and further downstream methods such as cell-subtype sorting and molecular biological approaches.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Experimental & Translational Stroke Medicine | ||||
| Publisher: | BMC | ||||
| ISSN: | 2040-7378 | ||||
| Official Date: | 2012 | ||||
| Dates: |
|
||||
| Volume: | 4 | ||||
| Number: | 1 | ||||
| Page Range: | p. 20 | ||||
| DOI: | 10.1186/2040-7378-4-20 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
