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Synthetically scalable poly(ampholyte) which dramatically enhances cellular cryopreservation
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Bailey, Trisha L., Stubbs, Christopher D., Murray, Kathryn, Tomás, Ruben M. F., Otten, Lucienne and Gibson, Matthew I. (2019) Synthetically scalable poly(ampholyte) which dramatically enhances cellular cryopreservation. Biomacromolecules, 20 (8). pp. 3104-3114. doi:10.1021/acs.biomac.9b00681 ISSN 1525-7797.
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WRAP-synthetically-scalable-polyampholyte-dramatically-enhances-cellular-cryopreservation-gibson-2019.pdf - Accepted Version - Requires a PDF viewer. Download (4014Kb) | Preview |
Official URL: http://dx.doi.org/10.1021/acs.biomac.9b00681
Abstract
The storage and transport of frozen cells underpin the emerging/existing cell-based therapies and are used in every biomedical research lab globally. The current gold-standard cryoprotectant dimethyl sulfoxide (DMSO) does not give quantitative cell recovery in suspension or in two-dimensional (2D) or three-dimensional (3D) cell models, and the solvent and cell debris must be removed prior to application/transfusion. There is a real need to improve this 50-year-old method to underpin emerging regenerative and cell-based therapies. Here, we introduce a potent and synthetically scalable polymeric cryopreservation enhancer which is easily obtained in a single step from a low cost and biocompatible precursor, poly(methyl vinyl ether-alt-maleic anhydride). This poly(ampholyte) enables post-thaw recoveries of up to 88% for a 2D cell monolayer model compared to just 24% using conventional DMSO cryopreservation. The poly(ampholyte) also enables reduction of [DMSO] from 10 wt % to just 2.5 wt % in suspension cryopreservation, which can reduce the negative side effects and speed up post-thaw processing. After thawing, the cells have reduced membrane damage and faster growth rates compared to those without the polymer. The polymer appears to function by a unique extracellular mechanism by stabilization of the cell membrane, rather than by modulation of ice formation and growth. This new macromolecular cryoprotectant will find applications across basic and translational biomedical science and may improve the cold chain for cell-based therapies.
Item Type: | Journal Article | |||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Cryopreservation of organs, tissues, etc. , Cells -- Cryopreservation, Frozen erythrocytes , Polyampholytes | |||||||||||||||||||||
Journal or Publication Title: | Biomacromolecules | |||||||||||||||||||||
Publisher: | American Chemical Society | |||||||||||||||||||||
ISSN: | 1525-7797 | |||||||||||||||||||||
Official Date: | 12 August 2019 | |||||||||||||||||||||
Dates: |
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Volume: | 20 | |||||||||||||||||||||
Number: | 8 | |||||||||||||||||||||
Page Range: | pp. 3104-3114 | |||||||||||||||||||||
DOI: | 10.1021/acs.biomac.9b00681 | |||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||
Date of first compliant deposit: | 5 July 2019 | |||||||||||||||||||||
Date of first compliant Open Access: | 20 June 2020 | |||||||||||||||||||||
RIOXX Funder/Project Grant: |
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