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Placental syncytiotrophoblast-derived extracellular vesicles carry active NEP (Neprilysin) and are increased in preeclampsia
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Gill, Manjot, Motta-Mejia, Carolina, Kandzija, Neva, Cooke , William, Zhang, Wei, Cerdeira, Ana Sofia, Bastie, Claire C., Redman, Christopher W. and Vatish, Manu (2019) Placental syncytiotrophoblast-derived extracellular vesicles carry active NEP (Neprilysin) and are increased in preeclampsia. Hypertension, 73 (5). pp. 1112-1119. doi:10.1161/HYPERTENSIONAHA.119.12707 ISSN 0194-911X.
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WRAP-Placental-syncytiotrophoblast-extracellular-neprilysin-preeclampsia-Bastie-23019.pdf - Accepted Version - Requires a PDF viewer. Download (608Kb) | Preview |
Official URL: https://doi.org/10.1161/HYPERTENSIONAHA.119.12707
Abstract
NEP (neprilysin) is a widely expressed membrane-bound metalloprotease, which binds and cleaves a variety of peptides including vasodilators, natriuretics, and diuretics. Higher levels of NEP result in hypertension-a cardinal feature of the placental disease preeclampsia. Syncytiotrophoblast-derived extracellular vesicles (EVs), comprising microvesicles and exosomes, are released into the peripheral circulation in pregnancy and are postulated as a key mechanism coupling placental dysfunction and maternal phenotype in preeclampsia. We aimed to determine whether higher levels of active NEP are found in syncytiotrophoblast-derived EVs in preeclampsia compared with normal pregnancy. Using immunostaining and Western blotting, we first demonstrated that NEP levels are greater not only in preeclampsia placental tissue but also in syncytiotrophoblast-derived microvesicles and exosomes isolated from preeclampsia placentas ( P<0.05, n=5). We confirmed placental origin using antibody-coated magnetic beads to isolate NEP-bound vesicles, finding that they stain for placental alkaline phosphatase. NEP on syncytiotrophoblast-derived EVs is active and inhibited by thiorphan ( P<0.01, n=3; specific inhibitor). Syncytiotrophoblast-derived microvesicles, isolated from peripheral plasma, demonstrated higher NEP expression in preeclampsia using flow cytometry ( P<0.05, n=8). We isolated plasma exosomes using size-exclusion chromatography and showed greater NEP activity in preeclampsia ( P<0.05, n=8). These findings show that the placenta releases active NEP into the maternal circulation on syncytiotrophoblast-derived EVs, at significantly greater levels in preeclampsia. NEP has pathological roles in hypertension, heart failure, and amyloid deposition, all of which are features of preeclampsia. Circulating syncytiotrophoblast-derived EV-bound NEP thus may contribute to the pathogenesis of this disease.
| Item Type: | Journal Article | ||||||||
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| Subjects: | R Medicine > RG Gynecology and obstetrics | ||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Preeclampsia -- Risk factors -- Research, Hypertension in pregnancy, Pregnancy -- Complications, Placenta -- Physiology, Ribonucleases | ||||||||
| Journal or Publication Title: | Hypertension | ||||||||
| Publisher: | American Heart Association | ||||||||
| ISSN: | 0194-911X | ||||||||
| Official Date: | 1 May 2019 | ||||||||
| Dates: |
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| Volume: | 73 | ||||||||
| Number: | 5 | ||||||||
| Page Range: | pp. 1112-1119 | ||||||||
| DOI: | 10.1161/HYPERTENSIONAHA.119.12707 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Restricted or Subscription Access | ||||||||
| Copyright Holders: | Manu Vatish | ||||||||
| Date of first compliant deposit: | 11 July 2019 | ||||||||
| Date of first compliant Open Access: | 1 April 2020 | ||||||||
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