The Library
How does eDNA compare to traditional trapping? Detecting mosquito communities in South-African freshwater ponds
Tools
Krol, Louie, Van der Hoorn, Berry, Gorsich, Erin E., Trimbos, Krijn, Bodegom, Peter M. van and Schrama, Maarten (2019) How does eDNA compare to traditional trapping? Detecting mosquito communities in South-African freshwater ponds. Frontiers in Ecology and Evolution, 7 . 260. doi:10.3389/fevo.2019.00260 ISSN 2296-701X.
|
PDF
WRAP-how-does-cDNA-compare-traditional-mosquito-communities-ponds-Gorsich-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1948Kb) | Preview |
Official URL: http://dx.doi.org/10.3389/fevo.2019.00260
Abstract
Improved biomonitoring of mosquitoes requires an in-depth understanding on occurrences of both vector and non-vector species, in larval, and adult stages. Accurate descriptions of the ecological context in which mosquitoes thrive remain limited, particularly for larval stages. The aim of this study was to develop a mixed-amplicon eDNA approach to assess (i) whether mosquito larval communities of stagnant fresh-water bodies can be detected using a Culicidae-specific primer and (ii) how these results compare to traditional trapping of adult mosquitoes. Results from 32 ponds inside and outside Kruger National Park, South Africa show that our primer detected mosquito eDNA. However, it yielded only a subset of the species found using adult trapping methods. Particularly the less frequent and container-breeding species were not found. Our approach provides the first steps toward an eDNA-based method to assess the entire community of larval-stage mosquitoes. It may thereby overcome current taxonomic hurdles presented by morphological identification of larvae. As such, it holds great promise for biomonitoring and ecological studies of mosquitoes.
Item Type: | Journal Article | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history Q Science > QL Zoology Q Science > QP Physiology R Medicine > RA Public aspects of medicine |
||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
Library of Congress Subject Headings (LCSH): | DNA , Environmental monitoring, Mosquitoes as carriers of disease, Mosquitoes as carriers of disease -- Detection -- South Africa, Mosquitoes -- Control | ||||||||||||
Journal or Publication Title: | Frontiers in Ecology and Evolution | ||||||||||||
Publisher: | Frontiers Media | ||||||||||||
ISSN: | 2296-701X | ||||||||||||
Official Date: | 10 July 2019 | ||||||||||||
Dates: |
|
||||||||||||
Volume: | 7 | ||||||||||||
Article Number: | 260 | ||||||||||||
DOI: | 10.3389/fevo.2019.00260 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
Date of first compliant deposit: | 23 July 2019 | ||||||||||||
Date of first compliant Open Access: | 24 July 2019 | ||||||||||||
RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year