Recovery efficiencies of nucleic acid extraction kits as measured by quantitative LightCycler (TM) PCR
UNSPECIFIED. (2001) Recovery efficiencies of nucleic acid extraction kits as measured by quantitative LightCycler (TM) PCR. JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, 54 (2). pp. 86-90. ISSN 1366-8714Full text not available from this repository.
Aims-To compare the efficiency of five nucleic acid extraction kits for processing clinical material for the diagnosis of infection.
Methods-Five nucleic acid extraction kits for processing clinical material for the diagnosis of infection were compared for their relative efficiencies in purifying and recovering either viral DNA or RNA from serum samples. Quantitative polymerase chain reaction (PCR) assays for hepatitis B virus (HBV) DNA and hepatitis C virus (HCV) RNA were performed on the Light-Cycler(TM) instrument to determine the relative concentrations of the viral nucleic acids recovered by the various protocols.
Results-Large differences between kits in recovery efficiencies were observed for HBV DNA, with those protocols using enzyme digestion in addition to chaotropic solutions performing better than those using chemical disruption alone.
Conclusions-There were large differences between the kits and it appeared that those extraction kits containing a proteolytic enzyme and carrier nucleic acid land that are supplied RNase free) have the widest potential application in the routine microbiology laboratory.
|Item Type:||Journal Article|
|Subjects:||R Medicine > RB Pathology|
|Journal or Publication Title:||JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY|
|Publisher:||BRITISH MED JOURNAL PUBL GROUP|
|Official Date:||April 2001|
|Number of Pages:||5|
|Page Range:||pp. 86-90|
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