High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector
UNSPECIFIED. (2001) High-level expression of bovine rotavirus VP4 protein in Escherichia coli using a hepatitis B core antigen vector. Biotechnology Letters, 23 (8). pp. 643-646. ISSN 0141-5492Full text not available from this repository.
Six regions of the VP4 protein of bovine rotavirus strain UKtc were expressed using hepatitis B core antigen (HBcAg) as a carrier. Following induction by IPTG, the six fusion proteins, AHBcAg through FHBcAg, were expressed in Escherichia coli to a level of 20-37% of total cellular protein. Soluble fusion proteins in a particulate form were partially purified with yields ranging from 0.5-6.4 mg l(-1) of culture.
|Item Type:||Journal Article|
|Subjects:||T Technology > TP Chemical technology|
|Journal or Publication Title:||Biotechnology Letters|
|Publisher:||KLUWER ACADEMIC PUBL|
|Official Date:||April 2001|
|Number of Pages:||4|
|Page Range:||pp. 643-646|
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