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Quantifying microsecond exchange in large protein complexes with accelerated relaxation dispersion experiments in the solid state
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Öster, Carl, Kosol, Simone and Lewandowski, Józef R. (2019) Quantifying microsecond exchange in large protein complexes with accelerated relaxation dispersion experiments in the solid state. Scientific Reports, 9 (11082). 11082 . doi:10.1038/s41598-019-47507-8 ISSN 2045-2322.
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Official URL: https://doi.org/10.1038/s41598-019-47507-8
Abstract
Solid state nMR is a powerful method to obtain information on the structure and dynamics of protein complexes that, due to solubility and size limitations, cannot be achieved by other methods. Here, we present an approach that allows the quantification of microsecond conformational exchange in large protein complexes by using a paramagnetic agent to accelerate 15n R1ρ relaxation dispersion measurements and overcome sensitivity limitations. The method is validated on crystalline GB1 and then applied to a >300 kDa precipitated complex of GB1 with full length human immunoglobulin G (igG). the addition of a paramagnetic agent increased the signal to noise ratio per time unit by a factor of 5, which allowed full relaxation dispersion curves to be recorded on a sample containing less than 50 μg of labelled material in 5 and 10 days on 850 and 700 MHz spectrometers, respectively. We discover a similar exchange process across the β-sheet in GB1 in crystals and in complex with IgG. However, the slow motion observed for a number of residues in the α-helix of crystalline GB1 is not detected in the complex.
Item Type: | Journal Article | |||||||||||||||||||||||||||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Proteins -- Analysis, Nuclear magnetic resonance spectroscopy | |||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Scientific Reports | |||||||||||||||||||||||||||||||||||||||
Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||||||||||||||
ISSN: | 2045-2322 | |||||||||||||||||||||||||||||||||||||||
Official Date: | 31 July 2019 | |||||||||||||||||||||||||||||||||||||||
Dates: |
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Volume: | 9 | |||||||||||||||||||||||||||||||||||||||
Number: | 11082 | |||||||||||||||||||||||||||||||||||||||
Number of Pages: | 10 | |||||||||||||||||||||||||||||||||||||||
Article Number: | 11082 | |||||||||||||||||||||||||||||||||||||||
DOI: | 10.1038/s41598-019-47507-8 | |||||||||||||||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||||||||||||||
Date of first compliant deposit: | 1 August 2019 | |||||||||||||||||||||||||||||||||||||||
Date of first compliant Open Access: | 1 August 2019 | |||||||||||||||||||||||||||||||||||||||
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