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Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms.
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Slater, Ross T., Frost, Lucy R., Jossi, Sian E, Millard, Andrew D. and Unnikrishnan, Meera (2019) Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms. Scientific Reports, 9 (1). 9903. doi:10.1038/s41598-019-46143-6
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WRAP-clostridioides-difficile-mediates-inter-bacterial-biofilm-Unnikrishnan-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2863Kb) | Preview |
Official URL: https://doi.org/10.1038/s41598-019-46143-6
Abstract
The anaerobic gut pathogen, Clostridioides difficile, forms adherent biofilms that may play an important role in recurrent C. difficile infections. The mechanisms underlying C. difficile community formation and inter-bacterial interactions are nevertheless poorly understood. C. difficile produces AI-2, a quorum sensing molecule that modulates biofilm formation across many bacterial species. We found that a strain defective in LuxS, the enzyme that mediates AI-2 production, is defective in biofilm development in vitro. Transcriptomic analyses of biofilms formed by wild type (WT) and luxS mutant (luxS) strains revealed a downregulation of prophage loci in the luxS mutant biofilms compared to the WT. Detection of phages and eDNA within biofilms may suggest that DNA release by phage-mediated cell lysis contributes to C. difficile biofilm formation. In order to understand if LuxS mediates C. difficile crosstalk with other gut species, C. difficile interactions with a common gut bacterium, Bacteroides fragilis, were studied. We demonstrate that C. difficile growth is significantly reduced when co-cultured with B. fragilis in mixed biofilms. Interestingly, the absence of C. difficile LuxS alleviates the B. fragilis-mediated growth inhibition. Dual species RNA-sequencing analyses from single and mixed biofilms revealed differential modulation of distinct metabolic pathways for C. difficile WT, luxS and B. fragilis upon co-culture, indicating that AI-2 may be involved in induction of selective metabolic responses in B. fragilis. Overall, our data suggest that C. difficile LuxS/AI-2 utilises different mechanisms to mediate formation of single and mixed species communities.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QR Microbiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Clostridium diseases, Clostridium diseases -- Prevention, Anaerobic infections , Biofilms | |||||||||
| Journal or Publication Title: | Scientific Reports | |||||||||
| Publisher: | Nature Publishing Group | |||||||||
| ISSN: | 2045-2322 | |||||||||
| Official Date: | 9 July 2019 | |||||||||
| Dates: |
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| Date of first compliant deposit: | 20 September 2019 | |||||||||
| Volume: | 9 | |||||||||
| Number: | 1 | |||||||||
| Article Number: | 9903 | |||||||||
| DOI: | 10.1038/s41598-019-46143-6 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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