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Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms.

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Slater, Ross T., Frost, Lucy R., Jossi, Sian E, Millard, Andrew D. and Unnikrishnan, Meera (2019) Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms. Scientific Reports, 9 (1). 9903. doi:10.1038/s41598-019-46143-6

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Official URL: https://doi.org/10.1038/s41598-019-46143-6

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Abstract

The anaerobic gut pathogen, Clostridioides difficile, forms adherent biofilms that may play an important role in recurrent C. difficile infections. The mechanisms underlying C. difficile community formation and inter-bacterial interactions are nevertheless poorly understood. C. difficile produces AI-2, a quorum sensing molecule that modulates biofilm formation across many bacterial species. We found that a strain defective in LuxS, the enzyme that mediates AI-2 production, is defective in biofilm development in vitro. Transcriptomic analyses of biofilms formed by wild type (WT) and luxS mutant (luxS) strains revealed a downregulation of prophage loci in the luxS mutant biofilms compared to the WT. Detection of phages and eDNA within biofilms may suggest that DNA release by phage-mediated cell lysis contributes to C. difficile biofilm formation. In order to understand if LuxS mediates C. difficile crosstalk with other gut species, C. difficile interactions with a common gut bacterium, Bacteroides fragilis, were studied. We demonstrate that C. difficile growth is significantly reduced when co-cultured with B. fragilis in mixed biofilms. Interestingly, the absence of C. difficile LuxS alleviates the B. fragilis-mediated growth inhibition. Dual species RNA-sequencing analyses from single and mixed biofilms revealed differential modulation of distinct metabolic pathways for C. difficile WT, luxS and B. fragilis upon co-culture, indicating that AI-2 may be involved in induction of selective metabolic responses in B. fragilis. Overall, our data suggest that C. difficile LuxS/AI-2 utilises different mechanisms to mediate formation of single and mixed species communities.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
R Medicine > RC Internal medicine
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection
Faculty of Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Clostridium diseases, Clostridium diseases -- Prevention, Anaerobic infections , Biofilms
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group
ISSN: 2045-2322
Official Date: 9 July 2019
Dates:
DateEvent
9 July 2019Published
21 June 2019Accepted
Volume: 9
Number: 1
Article Number: 9903
DOI: 10.1038/s41598-019-46143-6
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
105627/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100010269
UNSPECIFIED[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
Related URLs:
  • http://europepmc.org/articles/PMC6616478...
  • http://europepmc.org/articles/PMC6616478
  • https://doi.org/10.1038/s41598-019-46143...

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