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A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments
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Jones, Helen J., Kröber, Eileen, Stephenson, Jason, Mausz, Michaela A., Jameson, Eleanor, Millard, Andrew D., Purdy, Kevin J. and Chen, Yin (2019) A new family of uncultivated bacteria involved in methanogenesis from the ubiquitous osmolyte glycine betaine in coastal saltmarsh sediments. Microbiome, 7 . 120. doi:10.1186/s40168-019-0732-4 ISSN 2049-2618.
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Official URL: https://doi.org/10.1186/s40168-019-0732-4
Abstract
Background:
Coastal environments are dynamic and rapidly changing. Living organisms in coastal environments are known to synthesise large quantities of organic osmolytes, which they use to cope with osmotic stresses. The organic osmolyte glycine betaine (GBT) is ubiquitously found in marine biota from prokaryotic Bacteria and Archaea to coastal plants, marine protozoa, and mammals. In intertidal coastal sediment, GBT represents an important precursor of natural methane emissions and as much as 90% of total methane production in these ecosystems can be originated from methanogenesis from GBT and its intermediate trimethylamine through microbial metabolism.
Results:
We set out to uncover the microorganisms responsible for methanogenesis from GBT using stable isotope labelling and metagenomics. This led to the recovery of a near-complete genome (2.3 Mbp) of a novel clostridial bacterium involved in anaerobic GBT degradation. Phylogenetic analyses of 16S rRNA gene, functional marker genes, and comparative genomics analyses all support the establishment of a novel family Candidatus ‘Betainaceae’ fam. nov. in Clostridiales and its role in GBT metabolism.
Conclusions:
Our comparative genomes and metagenomics analyses suggest that this bacterium is widely distributed in coastal salt marshes, marine sediments, and deep subsurface sediments, suggesting a key role of anaerobic GBT metabolism by this clostridial bacterium in these ecosystems.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history | ||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
| Library of Congress Subject Headings (LCSH): | Coastal ecology, Virulence (Microbiology), Pathogenic bacteria, Coasts, Salt marsh ecology | ||||||
| Journal or Publication Title: | Microbiome | ||||||
| Publisher: | BMC | ||||||
| ISSN: | 2049-2618 | ||||||
| Official Date: | 27 August 2019 | ||||||
| Dates: |
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| Volume: | 7 | ||||||
| Article Number: | 120 | ||||||
| DOI: | 10.1186/s40168-019-0732-4 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 12 August 2019 | ||||||
| Date of first compliant Open Access: | 14 August 2019 | ||||||
| RIOXX Funder/Project Grant: |
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