Ellis, R. John. (2001) Macromolecular crowding: an important but neglected aspect of the intracellular environment. Current Opinion in Structural Biology, 11 (1). pp. 114-119. ISSN 0959-440X

Full text not available from this repository.

Abstract

Biological macromolecules have evolved over billions of years to function inside cells, so it is not surprising that researchers studying the properties of such molecules, either in extracts or in purified form, take care to control factors that reflect the intracellular environment, such as pH, ionic strength and composition, redox potential and the concentrations of relevant metabolites and effector molecules. There is one universal aspect of the cellular interior, however, that is largely neglected - the fact that it is highly crowded with macromolecules. It is proposed that the addition of crowding agents should become as routine as controlling pH and ionic strength if we are to meet the objective of studying biological molecules under more physiologically relevant conditions.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Current Opinion in Structural Biology
Publisher:	Elsevier
ISSN:	0959-440X
Date:	February 2001
Volume:	11
Number:	1
Number of Pages:	6
Page Range:	pp. 114-119
Identification Number:	10.1016/S0959-440X(00)00239-6
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
URI:	http://wrap.warwick.ac.uk/id/eprint/12414

Data sourced from Thomson Reuters' Web of Knowledge

Request changes to a record

Actions (login required)

View Item