UNSPECIFIED (2001) Apoptosis in squamous cell carcinoma of the lung: correlation with survival and clinicopathological features. JOURNAL OF CLINICAL PATHOLOGY, 54 (2). pp. 111-115. ISSN 0021-9746

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Abstract

Aims-Apoptosis is recognised as a physiological mechanism for controlling cell numbers and its subversion is thought to contribute to carcinogenesis. The aims of this study were to measure the apoptotic index (AI) in a series of squamous cell carcinomas (SCCs) of the lung using standard histological staining and confirm this by immunohistology using an antibody to an apoptosis specific protein (ASP), and to seek to correlate the AI with clinicopathological parameters. Methods-Sections of 134 SCCs were stained by haematoxylin and eosin (H&E) for counting apoptotic bodies to determine the Al (number of apoptotic bodies/10 000 tumour cells); 26 of these were also stained with anti-ASP antibody and the proportion of ASP positive cells counted. Clinical data were obtained from hospital notes. Results-The mean Al obtained by H&E staining of all 134 SCCs was 30.3 (SD, 24.75). Anti-ASP staining allowed identification of apoptotic bodies, generated a somewhat higher index (mean, 51.4; SD 39); this was not a result of the selection of tumours because the Al by H&E in the subset stained with anti-ASP was 31.1. Regression analysis showed that the correlation between the two values of AT was highly significant (Rs = 0.9760; p < 0.001), indicating that the two methods were both reliable measures of apoptosis but that the anti-ASP staining is the more sensitive method. The tumours were grouped into high AT (> 50) and low Al (< 50) and survival analysis was carried out. The mean survival of the high AT group was 109 weeks and of the low Al group 72 weeks (p = 0.036). Conclusions-Anti-ASP staining is a reliable, easy, and sensitive method for assessing apoptosis in tumour sections and confirms the validity of the AI obtained by H&E staining. Al is a guide to the behaviour of SCCs of the lung.

Item Type:	Journal Article
Subjects:	R Medicine > RB Pathology
Journal or Publication Title:	JOURNAL OF CLINICAL PATHOLOGY
Publisher:	BRITISH MED JOURNAL PUBL GROUP
ISSN:	0021-9746
Date:	February 2001
Volume:	54
Number:	2
Number of Pages:	5
Page Range:	pp. 111-115
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/12466

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