UNSPECIFIED (2001) Structure of the maize streak virus geminate particle. VIROLOGY, 279 (2). pp. 471-477. doi:10.1006/viro.2000.0739

Research output not available from this repository, contact author.

Request Changes to record.

Abstract

lThe Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstruction methods. The particle, of dimensions 220 x 380 Angstrom, has an overall 52-point-group symmetry, in which each half particle "head" consists of the coal protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel beta -barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal alpha -helix. This has produced a model of the geminate particle in which 110 copies of the CP nicely fit into the reconstructed density map. The reconstructed density map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure. (C) 2001 Academic Press.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	VIROLOGY
Publisher:	ACADEMIC PRESS INC
ISSN:	0042-6822
Official Date:	20 January 2001
Dates:	Date Event 20 January 2001 UNSPECIFIED
Volume:	279
Number:	2
Number of Pages:	7
Page Range:	pp. 471-477
DOI:	10.1006/viro.2000.0739
Publication Status:	Published

Data sourced from Thomson Reuters' Web of Knowledge

Request changes or add full text files to a record

Repository staff actions (login required)

View Item