UNSPECIFIED. (2001) Structure of the maize streak virus geminate particle. VIROLOGY, 279 (2). pp. 471-477. ISSN 0042-6822

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Abstract

lThe Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or "geminate" particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstruction methods. The particle, of dimensions 220 x 380 Angstrom, has an overall 52-point-group symmetry, in which each half particle "head" consists of the coal protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel beta -barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal alpha -helix. This has produced a model of the geminate particle in which 110 copies of the CP nicely fit into the reconstructed density map. The reconstructed density map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure. (C) 2001 Academic Press.

Item Type:	Journal Article
Subjects:	Q Science > QR Microbiology > QR355 Virology
Journal or Publication Title:	VIROLOGY
Publisher:	ACADEMIC PRESS INC
ISSN:	0042-6822
Official Date:	20 January 2001
Volume:	279
Number:	2
Number of Pages:	7
Page Range:	pp. 471-477
Identification Number:	10.1006/viro.2000.0739
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/12474

Data sourced from Thomson Reuters' Web of Knowledge

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