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Structural basis of glycerophosphodiester recognition by the mycobacterium tuberculosis substrate-binding protein UgpB

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Fenn, Jonathan S., Nepravishta, Ridvan, Guy, Collette S., Harrison, James, Angulo, Jesus, Cameron, Alexander and Fullam, Elizabeth (2019) Structural basis of glycerophosphodiester recognition by the mycobacterium tuberculosis substrate-binding protein UgpB. ACS Chemical Biology, 14 (9). pp. 1879-1887. doi:10.1021/acschembio.9b00204 ISSN 1554-8929.

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Official URL: http://dx.doi.org/10.1021/acschembio.9b00204

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Abstract

Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB) and has evolved an incredible ability to survive latently within the human host for decades. The Mtb pathogen encodes for a low number of ATP-binding cassette (ABC) importers for the acquisition of carbohydrates that may reflect the nutrient poor environment within the host macrophages. Mtb UgpB (Rv2833c) is the substrate binding domain of the UgpABCE transporter that recognizes glycerophosphocholine (GPC), indicating that this transporter has a role in recycling glycerophospholipid metabolites. By using a combination of saturation transfer difference (STD) NMR and X-ray crystallography, we report the structural analysis of Mtb UgpB complexed with GPC and have identified that Mtb UgpB not only recognizes GPC but is also promiscuous for a broad range of glycerophosphodiesters. Complementary biochemical analyses and site-directed mutagenesis precisely define the molecular basis and specificity of glycerophosphodiester recognition. Our results provide critical insights into the structural and functional role of the Mtb UgpB transporter and reveal that the specificity of this ABC-transporter is not limited to GPC, therefore optimizing the ability of Mtb to scavenge scarce nutrients and essential glycerophospholipid metabolites via a single transporter during intracellular infection.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Q Science > QR Microbiology
R Medicine > RC Internal medicine
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Mycobacterium tuberculosis , Tuberculosis , Proteins -- Chemical modification, Protein binding, Ligands (Biochemistry)
Journal or Publication Title: ACS Chemical Biology
Publisher: American Chemical Society
ISSN: 1554-8929
Official Date: 21 August 2019
Dates:
DateEvent
21 August 2019Available
7 August 2019Accepted
Volume: 14
Number: 9
Page Range: pp. 1879-1887
DOI: 10.1021/acschembio.9b00204
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Copyright Holders: © 2019 American Chemical Society
Date of first compliant deposit: 2 September 2019
Date of first compliant Open Access: 2 September 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
104193/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100010269
104193/Z/14/ZRoyal Societyhttp://dx.doi.org/10.13039/501100000288
RG120405Royal Societyhttp://dx.doi.org/10.13039/501100000288
MR/J003964/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
EP/M027503/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/P010660/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

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