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Polyampholytes as emerging macromolecular cryoprotectants
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Stubbs, Christopher D., Bailey, Trisha L., Murray, Kathryn and Gibson, Matthew I. (2019) Polyampholytes as emerging macromolecular cryoprotectants. Biomacromolecules . doi:10.1021/acs.biomac.9b01053 (In Press)
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WRAP-polyampholytes-emerging-macromolecular-cryoprotectants-Stubbs-2019.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2602Kb) | Preview |
Official URL: https://doi.org/10.1021/acs.biomac.9b01053
Abstract
Cellular cryopreservation is a platform technology which underpins cell biology, biochemistry, biomaterials, diagnostics, and the cold chain for emerging cell-based therapies. This technique relies on effective methods for banking and shipping to avoid the need for continuous cell culture. The most common method to achieve cryopreservation is to use large volumes of organic solvent cryoprotective agents which can promote either a vitreous (ice free) phase or dehydrate and protect the cells. These methods are very successful but are not perfect: not all cell types can be cryopreserved and recovered, and the cells do not always retain their phenotype and function post-thaw. This Perspective will introduce polyampholytes as emerging macromolecular cryoprotective agents and demonstrate they have the potential to impact a range of fields from cell-based therapies to basic cell biology and may be able to improve, or replace, current solvent-based cryoprotective agents. Polyampholytes have been shown to be remarkable (mammalian cell) cryopreservation enhancers, but their mechanism of action is unclear, which may include membrane protection, solvent replacement, or a yet unknown protective mechanism, but it seems the modulation of ice growth (recrystallization) may only play a minor role in their function, unlike other macromolecular cryoprotectants. This Perspective will discuss their synthesis and summarize the state-of-the-art, including hypotheses of how they function, to introduce this exciting area of biomacromolecular science.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history |
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| Divisions: | Faculty of Science > Chemistry | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Polyampholytes, Cells -- Cryopreservation | |||||||||
| Journal or Publication Title: | Biomacromolecules | |||||||||
| Publisher: | American Chemical Society (ACS) | |||||||||
| ISSN: | 1526-4602 | |||||||||
| Official Date: | 27 August 2019 | |||||||||
| Dates: |
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| Date of first compliant deposit: | 13 September 2019 | |||||||||
| DOI: | 10.1021/acs.biomac.9b01053 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | In Press | |||||||||
| Publisher Statement: | ** Article version: VoR ** From Crossref via Jisc Publications Router ** History: epub 27-08-2019; issued 27-08-2019. ** Licence for VoR version of this article starting on 27-08-2019: http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| Copyright Holders: | Copyright © 2019 American Chemical Society | |||||||||
| RIOXX Funder/Project Grant: |
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