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Polyampholytes as emerging macromolecular cryoprotectants

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Stubbs, Christopher D., Bailey, Trisha L., Murray, Kathryn and Gibson, Matthew I. (2020) Polyampholytes as emerging macromolecular cryoprotectants. Biomacromolecules, 21 (1). pp. 7-17. doi:10.1021/acs.biomac.9b01053

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Official URL: https://doi.org/10.1021/acs.biomac.9b01053

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Abstract

Cellular cryopreservation is a platform technology which underpins cell biology, biochemistry, biomaterials, diagnostics, and the cold chain for emerging cell-based therapies. This technique relies on effective methods for banking and shipping to avoid the need for continuous cell culture. The most common method to achieve cryopreservation is to use large volumes of organic solvent cryoprotective agents which can promote either a vitreous (ice free) phase or dehydrate and protect the cells. These methods are very successful but are not perfect: not all cell types can be cryopreserved and recovered, and the cells do not always retain their phenotype and function post-thaw. This Perspective will introduce polyampholytes as emerging macromolecular cryoprotective agents and demonstrate they have the potential to impact a range of fields from cell-based therapies to basic cell biology and may be able to improve, or replace, current solvent-based cryoprotective agents. Polyampholytes have been shown to be remarkable (mammalian cell) cryopreservation enhancers, but their mechanism of action is unclear, which may include membrane protection, solvent replacement, or a yet unknown protective mechanism, but it seems the modulation of ice growth (recrystallization) may only play a minor role in their function, unlike other macromolecular cryoprotectants. This Perspective will discuss their synthesis and summarize the state-of-the-art, including hypotheses of how they function, to introduce this exciting area of biomacromolecular science.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
Divisions: Faculty of Science > Chemistry
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Polyampholytes, Cells -- Cryopreservation
Journal or Publication Title: Biomacromolecules
Publisher: American Chemical Society (ACS)
ISSN: 1526-4602
Official Date: 13 January 2020
Dates:
DateEvent
13 January 2020Published
16 August 2019Available
12 August 2019Accepted
Volume: 21
Number: 1
Page Range: pp. 7-17
DOI: 10.1021/acs.biomac.9b01053
Status: Peer Reviewed
Publication Status: Published
Publisher Statement: ** Article version: VoR ** From Crossref via Jisc Publications Router ** History: epub 27-08-2019; issued 27-08-2019. ** Licence for VoR version of this article starting on 27-08-2019: http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html
Access rights to Published version: Open Access
Copyright Holders: Copyright © 2019 American Chemical Society
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
638661European Research Councilhttp://dx.doi.org/10.13039/501100000781
PoC Grant 789182European Research Councilhttp://dx.doi.org/10.13039/501100000781
Related URLs:
  • http://pubs.acs.org/page/policy/authorch...

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