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The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells
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Vlisidou, Isabella, Hapeshi, Alexia, Healey, Joseph, Smart, Katie, Yang, Guowei and Waterfield, Nicholas R. (2019) The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells. eLife, 8 . e46259.. doi:10.7554/eLife.46259
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Official URL: https://doi.org/10.7554/eLife.46259
Abstract
Photorhabdus is a highly effective insect pathogen and symbiont of insecticidal nematodes. To exert its potent insecticidal effects, it elaborates a myriad of toxins and small molecule effectors. Among these, the Photorhabdus Virulence Cassettes (PVCs) represent an elegant self-contained delivery mechanism for diverse protein toxins. Importantly, these self-contained nanosyringes overcome host cell membrane barriers, and act independently, at a distance from the bacteria itself. In this study, we demonstrate that Pnf, a PVC needle complex associated toxin, is a Rho-GTPase, which acts via deamidation and transglutamination to disrupt the cytoskeleton. TEM and Western blots have shown a physical association between Pnf and its cognate PVC delivery mechanism. We demonstrate that for Pnf to exert its effect, translocation across the cell membrane is absolutely essential.
| Item Type: | Journal Article | ||||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology | ||||||
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Medicine > Warwick Medical School |
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| Journal or Publication Title: | eLife | ||||||
| Publisher: | eLife Sciences Publications Ltd. | ||||||
| ISSN: | 2050-084X | ||||||
| Official Date: | 17 September 2019 | ||||||
| Dates: |
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| Date of first compliant deposit: | 12 September 2019 | ||||||
| Volume: | 8 | ||||||
| Article Number: | e46259. | ||||||
| DOI: | 10.7554/eLife.46259 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access | ||||||
| Funder: | Leverhulme Trust, BBSRC | ||||||
| Grant number: | BB/C008367/1, RPG-2015-194 |
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