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The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells

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Vlisidou, Isabella, Hapeshi, Alexia, Healey, Joseph R. J., Smart, Katie, Yang, Guowei and Waterfield, Nicholas R. (2019) The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells. eLife, 8 . e46259.. doi:10.7554/eLife.46259

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Official URL: https://doi.org/10.7554/eLife.46259

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Abstract

Photorhabdus is a highly effective insect pathogen and symbiont of insecticidal nematodes. To exert its potent insecticidal effects, it elaborates a myriad of toxins and small molecule effectors. Among these, the Photorhabdus Virulence Cassettes (PVCs) represent an elegant self-contained delivery mechanism for diverse protein toxins. Importantly, these self-contained nanosyringes overcome host cell membrane barriers, and act independently, at a distance from the bacteria itself. In this study, we demonstrate that Pnf, a PVC needle complex associated toxin, is a Rho-GTPase, which acts via deamidation and transglutamination to disrupt the cytoskeleton. TEM and Western blots have shown a physical association between Pnf and its cognate PVC delivery mechanism. We demonstrate that for Pnf to exert its effect, translocation across the cell membrane is absolutely essential.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
R Medicine > RA Public aspects of medicine
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Pathogenic bacteria , Proteins -- Structure, Vector-pathogen relationships, Eukaryotic cells
Journal or Publication Title: eLife
Publisher: eLife Sciences Publications Ltd.
ISSN: 2050-084X
Official Date: 17 September 2019
Dates:
DateEvent
17 September 2019Published
12 June 2019Accepted
Date of first compliant deposit: 12 September 2019
Volume: 8
Article Number: e46259.
DOI: 10.7554/eLife.46259
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/C008367/1 [BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
RPG-2015-194Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
DTPEP/F500378/1 [EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
UNSPECIFIEDUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
MR/N014294/1 [MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
BB/E021328/[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

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