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The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells
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Vlisidou, Isabella, Hapeshi, Alexia, Healey, Joseph R. J., Smart, Katie, Yang, Guowei and Waterfield, Nicholas R. (2019) The PVC element of Photorhabdus asymbiotica virulence cassettes deliver protein effectors directly into target eukaryotic cells. eLife, 8 . e46259.. doi:10.7554/eLife.46259 ISSN 2050-084X.
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Official URL: https://doi.org/10.7554/eLife.46259
Abstract
Photorhabdus is a highly effective insect pathogen and symbiont of insecticidal nematodes. To exert its potent insecticidal effects, it elaborates a myriad of toxins and small molecule effectors. Among these, the Photorhabdus Virulence Cassettes (PVCs) represent an elegant self-contained delivery mechanism for diverse protein toxins. Importantly, these self-contained nanosyringes overcome host cell membrane barriers, and act independently, at a distance from the bacteria itself. In this study, we demonstrate that Pnf, a PVC needle complex associated toxin, is a Rho-GTPase, which acts via deamidation and transglutamination to disrupt the cytoskeleton. TEM and Western blots have shown a physical association between Pnf and its cognate PVC delivery mechanism. We demonstrate that for Pnf to exert its effect, translocation across the cell membrane is absolutely essential.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology R Medicine > RA Public aspects of medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Pathogenic bacteria , Proteins -- Structure, Vector-pathogen relationships, Eukaryotic cells | |||||||||||||||||||||
| Journal or Publication Title: | eLife | |||||||||||||||||||||
| Publisher: | eLife Sciences Publications Ltd. | |||||||||||||||||||||
| ISSN: | 2050-084X | |||||||||||||||||||||
| Official Date: | 17 September 2019 | |||||||||||||||||||||
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| Volume: | 8 | |||||||||||||||||||||
| Article Number: | e46259. | |||||||||||||||||||||
| DOI: | 10.7554/eLife.46259 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 12 September 2019 | |||||||||||||||||||||
| Date of first compliant Open Access: | 17 September 2019 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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