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Data for Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces

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Ishibe, Toru, Congdon, Thomas R., Stubbs, Christopher D., Hasan, Muhammad, Sosso, Gabriele C. and Gibson, Matthew I. (2019) Data for Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces. [Dataset]

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Official URL: http://dx.doi.org/10.1021/acsmacrolett.9b00386

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Abstract

Antifreeze (glyco) proteins (AF(G)Ps) are potent inhibitors of ice recrystallization and may have biotechnological applications. The most potent AF(G)Ps function at concentrations a thousand times lower than synthetic mimics such as poly(vinyl alcohol), PVA. Here, we demonstrate that PVA’s ice recrystallization activity can be rescued at concentrations where it does not normally function, by the addition of noninteracting polymeric depletants, due to PVA forming colloids in the concentrated saline environment present between ice crystals. These depletants shift the equilibrium toward ice binding and, hence, enable PVA to inhibit ice growth at lower concentrations. Using theory and experiments, we show this effect requires polymeric depletants, not small molecules, to enhance activity. These results increase our understanding of how to design new ice growth inhibitors, but also offer opportunities to enhance activity by exploiting depletion forces, without re-engineering ice-binding materials. It also shows that when screening for IRI activity that polymer contaminants in buffers may give rise to false positive results.

Item Type: Dataset
Subjects: Q Science > QC Physics
Q Science > QP Physiology
Divisions: Faculty of Science > Chemistry
Faculty of Medicine > Warwick Medical School
Type of Data: Experimental data in xlsx format
Library of Congress Subject Headings (LCSH): Antifreeze proteins, Ice crystals -- Growth
Journal or Publication Title: ACS Macro Letters
Publisher: Department of Chemistry, University of Warwick
ISSN: 2161-1653
Official Date: 12 August 2019
Dates:
DateEvent
12 August 2019Available
Volume: 8
Number: 8
Page Range: pp. 1063-1067
DOI: 10.1021/acsmacrolett.9b00386
Status: Not Peer Reviewed
Publication Status: Published
Media of Output: .xlsx
Access rights to Published version: Open Access
Copyright Holders: University of Warwick
Description:

Size Exclusion Chromatography (SEC) data in xlsx format.
Raw data for figures in related article (Figures 2A, 2B, 2G, 3A, 4).
Raw data for figures in related Supplementary Information (Figures S1A, S1B, S1C, S1D, S1E, S1F, S2, S3)

RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
638661European Research Councilhttp://dx.doi.org/10.13039/501100000781
PoC Grant (789182)European Research Councilhttp://dx.doi.org/10.13039/501100000781
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Contributors:
ContributionNameContributor ID
DepositorGibson, Matthew I.32015

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