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Data for Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces
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Ishibe, Toru, Congdon, Thomas R., Stubbs, Christopher D., Hasan, Muhammad, Sosso, Gabriele C. and Gibson, Matthew I. (2019) Data for Enhancement of macromolecular ice recrystallization inhibition activity by exploiting depletion forces. [Dataset]
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Official URL: http://dx.doi.org/10.1021/acsmacrolett.9b00386
Abstract
Antifreeze (glyco) proteins (AF(G)Ps) are potent inhibitors of ice recrystallization and may have biotechnological applications. The most potent AF(G)Ps function at concentrations a thousand times lower than synthetic mimics such as poly(vinyl alcohol), PVA. Here, we demonstrate that PVA’s ice recrystallization activity can be rescued at concentrations where it does not normally function, by the addition of noninteracting polymeric depletants, due to PVA forming colloids in the concentrated saline environment present between ice crystals. These depletants shift the equilibrium toward ice binding and, hence, enable PVA to inhibit ice growth at lower concentrations. Using theory and experiments, we show this effect requires polymeric depletants, not small molecules, to enhance activity. These results increase our understanding of how to design new ice growth inhibitors, but also offer opportunities to enhance activity by exploiting depletion forces, without re-engineering ice-binding materials. It also shows that when screening for IRI activity that polymer contaminants in buffers may give rise to false positive results.
| Item Type: | Dataset | |||||||||
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| Subjects: | Q Science > QC Physics Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Chemistry Faculty of Medicine > Warwick Medical School |
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| Type of Data: | Experimental data in xlsx format | |||||||||
| Library of Congress Subject Headings (LCSH): | Antifreeze proteins, Ice crystals -- Growth | |||||||||
| Journal or Publication Title: | ACS Macro Letters | |||||||||
| Publisher: | Department of Chemistry, University of Warwick | |||||||||
| ISSN: | 2161-1653 | |||||||||
| Official Date: | 12 August 2019 | |||||||||
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| Date of first compliant deposit: | 21 August 2019 | |||||||||
| Volume: | 8 | |||||||||
| Number: | 8 | |||||||||
| Page Range: | pp. 1063-1067 | |||||||||
| DOI: | 10.1021/acsmacrolett.9b00386 | |||||||||
| Status: | Not Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| Copyright Holders: | University of Warwick | |||||||||
| Description: | Size Exclusion Chromatography (SEC) data in xlsx format. |
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