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Transcript abundance of Photorhabdus Insect-Related (Pir) toxin in Manduca sexta and Galleria mellonella infections

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Castagnola, Anaïs, Mulley, Geraldine, Davis, Nathaniel J. L. K., Waterfield, Nicholas R. and Stock, S Patricia (2016) Transcript abundance of Photorhabdus Insect-Related (Pir) toxin in Manduca sexta and Galleria mellonella infections. Toxins, 8 (10). 287. doi:10.3390/toxins8100287 ISSN 2072-6651.

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Official URL: https://doi.org/10.3390/toxins8100287

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Abstract

In this study, we assessed pirAB toxin transcription in (strain TT01) (Enterobacteriaceae) by comparing mRNA abundance under in vivo and in vitro conditions. In vivo assays considered both natural and forced infections with two lepidopteran hosts: and . Three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). We also assessed transcription during the course of a natural infection; this is when the bacteria are delivered by nematodes. Transcript abundance in was higher than in at two of the observed time points: 15 and 18 h. Expression of reached above endogenous control levels at 22 h in but not in . Overall, transcripts were not as highly expressed in as in from 15 to 22 h. This is the first study to directly compare toxin transcript production considering different portals of entry.

Item Type: Journal Article
Subjects: Q Science > QL Zoology
Q Science > QR Microbiology
S Agriculture > SB Plant culture
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Insect pests -- Biological control, Insect nematodes, Nematodes as carriers of disease, Nematodes as biological pest control agents, Insects -- Diseases
Journal or Publication Title: Toxins
Publisher: MDPI Publishing
ISSN: 2072-6651
Official Date: 29 September 2016
Dates:
DateEvent
29 September 2016Published
22 September 2016Accepted
6 June 2016Submitted
Volume: 8
Number: 10
Article Number: 287
DOI: 10.3390/toxins8100287
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 30 September 2019
Date of first compliant Open Access: 30 September 2019
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
1K12 GM 00708, IRACDA ProgramNational Institutes of Healthhttp://dx.doi.org/10.13039/100000002
NSF-IOS 0840932National Science Foundationhttp://dx.doi.org/10.13039/100000001

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