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Transcript abundance of Photorhabdus Insect-Related (Pir) toxin in Manduca sexta and Galleria mellonella infections
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Castagnola, Anaïs, Mulley, Geraldine, Davis, Nathaniel J. L. K., Waterfield, Nicholas R. and Stock, S Patricia (2016) Transcript abundance of Photorhabdus Insect-Related (Pir) toxin in Manduca sexta and Galleria mellonella infections. Toxins, 8 (10). 287. doi:10.3390/toxins8100287 ISSN 2072-6651.
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WRAP-transcript-abundance-Photorhabdus-Insect-Related-(Pir)-toxin-Manduca-sexta-Galleria-mellonella-infections-Waterfield-2016.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (1103Kb) | Preview |
Official URL: https://doi.org/10.3390/toxins8100287
Abstract
In this study, we assessed pirAB toxin transcription in (strain TT01) (Enterobacteriaceae) by comparing mRNA abundance under in vivo and in vitro conditions. In vivo assays considered both natural and forced infections with two lepidopteran hosts: and . Three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). We also assessed transcription during the course of a natural infection; this is when the bacteria are delivered by nematodes. Transcript abundance in was higher than in at two of the observed time points: 15 and 18 h. Expression of reached above endogenous control levels at 22 h in but not in . Overall, transcripts were not as highly expressed in as in from 15 to 22 h. This is the first study to directly compare toxin transcript production considering different portals of entry.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QL Zoology Q Science > QR Microbiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Insect pests -- Biological control, Insect nematodes, Nematodes as carriers of disease, Nematodes as biological pest control agents, Insects -- Diseases | |||||||||
| Journal or Publication Title: | Toxins | |||||||||
| Publisher: | MDPI Publishing | |||||||||
| ISSN: | 2072-6651 | |||||||||
| Official Date: | 29 September 2016 | |||||||||
| Dates: |
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| Volume: | 8 | |||||||||
| Number: | 10 | |||||||||
| Article Number: | 287 | |||||||||
| DOI: | 10.3390/toxins8100287 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 30 September 2019 | |||||||||
| Date of first compliant Open Access: | 30 September 2019 | |||||||||
| RIOXX Funder/Project Grant: |
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