UNSPECIFIED (2000) Mammalian meiotic telomeres: Protein composition and redistribution in relation to nuclear pores. MOLECULAR BIOLOGY OF THE CELL, 11 (12). pp. 4189-4203. ISSN 1059-1524

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Abstract

Mammalian telomeres consist of TTAGGG repeats, telomeric repeat binding factor (TRF), and other proteins, resulting in a protective structure at chromosome ends. Although structure and function of the somatic telomeric complex has been elucidated in some detail, the protein composition of mammalian meiotic telomeres is undetermined. Here we show, by indirect immunofluorescence (IF), that the meiotic telomere complex is similar to its somatic counterpart and contains significant amounts of TRF1, TRF2, and hRap1, while tankyrase, a poly-(ADP-ribose)polymerase at somatic telomeres and nuclear pores, forms small signals at ends of human meiotic chromosome cores. Analysis of rodent spermatocytes reveals Trf1 at mouse, TRF2 at rat, and mammalian Rap1 at meiotic telomeres of both rodents. Moreover, we demonstrate that telomere repositioning during meiotic prophase occurs in sectors of the nuclear envelope that are distinct from nuclear pore-dense areas. The latter form during preleptotene/leptotene and are present during entire prophase I.

Item Type:	Journal Article
Subjects:	Q Science > QH Natural history > QH301 Biology
Journal or Publication Title:	MOLECULAR BIOLOGY OF THE CELL
Publisher:	AMER SOC CELL BIOLOGY
ISSN:	1059-1524
Date:	December 2000
Volume:	11
Number:	12
Number of Pages:	15
Page Range:	pp. 4189-4203
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/12603

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