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The centriolar protein CPAP G-box : an amyloid fibril in a single domain
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Cutts, E. E., Inglis, A., Stansfeld, Phillip J., Vakonakis, I. and Hatzopoulos, G. N. (2015) The centriolar protein CPAP G-box : an amyloid fibril in a single domain. Biochemical Society Transactions, 43 (5). pp. 838-843. doi:10.1042/BST20150082 ISSN 0300-5127.
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Official URL: http://dx.doi.org/10.1042/BST20150082
Abstract
Centrioles are evolutionarily conserved cylindrical cell organelles with characteristic radial symmetry. Despite their considerable size (400 nm × 200 nm, in humans), genetic studies suggest that relatively few protein components are involved in their assembly. We recently characterized the molecular architecture of the centrosomal P4.1-associated protein (CPAP), which is crucial for controlling the centriolar cylinder length. Here, we review the remarkable architecture of the C-terminal domain of CPAP, termed the G-box, which comprises a single, entirely solvent exposed, antiparallel β-sheet. Molecular dynamics simulations support the stability of the G-box domain even in the face of truncations or amino acid substitutions. The similarity of the G-box domain to amyloids (or amyloid precursors) is strengthened by its oligomeric arrangement to form continuous fibrils. G-box fibrils were observed in crystals as well as in solution and are also supported by simulations. We conclude that the G-box domain may well represent the best analogue currently available for studies of exposed β-sheets, unencumbered by additional structural elements or severe aggregations problems.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Biochemical Society Transactions | ||||
Publisher: | Portland Press Ltd | ||||
ISSN: | 0300-5127 | ||||
Official Date: | October 2015 | ||||
Dates: |
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Volume: | 43 | ||||
Number: | 5 | ||||
Page Range: | pp. 838-843 | ||||
DOI: | 10.1042/BST20150082 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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