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Precise Measurement of Time Dependent Cell Growth and Detection of Partial Drug Resistance in Infectious Microbial
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Hu, Qingfeng, Yu, Yan, Gu, Dongshi, Xie, Li, Chen, Xingrou, Xu, Ning, Ruan, Jennifer Jin, Dowson, Christopher G. and Ruan, Benfang Helen (2019) Precise Measurement of Time Dependent Cell Growth and Detection of Partial Drug Resistance in Infectious Microbial. ACS Infectious Diseases, 5 (7). pp. 1252-1263. doi:10.1021/acsinfecdis.9b00132 ISSN 2373-8227.
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WRAP-detection-hidden-antimicrobial-drug-resistance-2019.pdf - Accepted Version - Requires a PDF viewer. Download (1848Kb) | Preview |
Official URL: https://doi.org/10.1021/acsinfecdis.9b00132
Abstract
Antimicrobial drug resistance has become a serious public health problem. The current clinical diagnostic methods are turbidity-based assays that have been used for years to track bacterial growth; however, the method is relatively insensitive. To eliminate the new occurrence of drug resistance in infectious bacteria, we developed a highly sensitive EZMTT method for the antibiotic susceptibility test (AST) that magnified the cell growth signal and revealed partial drug resistance (showing 2–20% weak cell growth) that was not detected by the current turbidity assay within 24 h. By simply mixing the EZMTT dye with the bacterial culture and then following the growth by absorbance measurement at 450 nm, the drug-induced proliferation (DIP) rate can be obtained in a high-throughput-screening (HTS) mode with greater than 10-fold better sensitivity than the turbidity assay. The EZMTT-based DIP rate assay of 5 clinically isolated E. coli strains found approximately 30% more partial drug resistance than what was detected in the traditional turbidity-based assay. The observed partial drug resistance was further confirmed by mechanistic analyses. Therefore, a combination of the EZMTT dye and the current clinically used VITEK-type technology has great potential to help understand antimicrobial drug resistance and ultimately provide patients with precise medical care to prevent the occurrence of multidrug resistant bacteria.
Item Type: | Journal Article | ||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QR Microbiology R Medicine > RA Public aspects of medicine R Medicine > RB Pathology R Medicine > RC Internal medicine |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
SWORD Depositor: | Library Publications Router | ||||||||||||
Library of Congress Subject Headings (LCSH): | Drug resistance, Communicable diseases , Infection , Cells -- Growth , Cells -- Effect of drugs on , Drug resistance in microorganisms | ||||||||||||
Journal or Publication Title: | ACS Infectious Diseases | ||||||||||||
Publisher: | American Chemical Society (ACS) | ||||||||||||
ISSN: | 2373-8227 | ||||||||||||
Official Date: | 12 July 2019 | ||||||||||||
Dates: |
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Volume: | 5 | ||||||||||||
Number: | 7 | ||||||||||||
Page Range: | pp. 1252-1263 | ||||||||||||
DOI: | 10.1021/acsinfecdis.9b00132 | ||||||||||||
Status: | Peer Reviewed | ||||||||||||
Publication Status: | Published | ||||||||||||
Reuse Statement (publisher, data, author rights): | “This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Infectious Diseases, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/page/policy/articlesonrequest/index.html | ||||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||||
Date of first compliant deposit: | 24 October 2019 | ||||||||||||
Date of first compliant Open Access: | 6 June 2020 | ||||||||||||
RIOXX Funder/Project Grant: |
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