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Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement
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Palani, Saravanan, Köster, Darius, Hatano, Tomoyuki, Kamnev, Anton, Kanamaru, Taishi, Brooker, Holly R., Hernandez-Fernaud, Juan Ramon, Jones, Alexandra M. E., Millar, Jonathan B. A., Mulvihill, Daniel P. and Balasubramanian, Mohan K. (2019) Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement. Journal of Cell Biology, 218 (11). pp. 3548-3559. doi:10.1083/jcb.201809089
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Official URL: http://dx.doi.org/10.1083/jcb.201809089
Abstract
Tropomyosin is a coiled-coil actin binding protein key to the stability of actin filaments. In muscle cells, tropomyosin is subject to calcium regulation, but its regulation in nonmuscle cells is not understood. Here, we provide evidence that the fission yeast tropomyosin, Cdc8, is regulated by phosphorylation of a serine residue. Failure of phosphorylation leads to an increased number and stability of actin cables and causes misplacement of the division site in certain genetic backgrounds. Phosphorylation of Cdc8 weakens its interaction with actin filaments. Furthermore, we show through in vitro reconstitution that phosphorylation-mediated release of Cdc8 from actin filaments facilitates access of the actin-severing protein Adf1 and subsequent filament disassembly. These studies establish that phosphorylation may be a key mode of regulation of nonmuscle tropomyosins, which in fission yeast controls actin filament stability and division site placement.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QP Physiology |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Tropomyosins, Biochemistry, Cell division, Cell cycle -- Research | ||||||||||||||||||
| Journal or Publication Title: | Journal of Cell Biology | ||||||||||||||||||
| Publisher: | Rockefeller University Press | ||||||||||||||||||
| ISSN: | 0021-9525 | ||||||||||||||||||
| Official Date: | 4 November 2019 | ||||||||||||||||||
| Dates: |
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| Volume: | 218 | ||||||||||||||||||
| Number: | 11 | ||||||||||||||||||
| Page Range: | pp. 3548-3559 | ||||||||||||||||||
| DOI: | 10.1083/jcb.201809089 | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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